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    A comparison of immuncapture agglutination and ELISA methods in serological diagnosis of brucellosis
    (Ivyspring International Publisher, 2011) Özdemir M.; Feyzio?lu B.; Kurto?lu M.G.; Do?an M.; Da?ï H.T.; Yüksekkaya S.; Keşli R.
    Background: Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic values of ELISA Ig M and Ig G and immuncapture agglutination tests with Coombs anti-brucella test. Methods: Sera from 200 patients with presumptive diagnosis of brucellosis were included into the study. Coombs anti-brucella test, ELISA Ig M and Ig G tests and Immuncapture test were investigated in these sera. Then, sensitivity, specificity, negative predictive and positive predictive values were calculated. Results: Sensitivity, specificity, negative predictive and positive predictive values were found to be 90,6 %, 76,3 %, 94,2 %, and 65,9 % respectively for the Immuncapture test, whereas they were found to be 73,7 %, 58,9 %, 84,2 %, and 42,8 % for Ig G and 72,2 %, 67,8 %, 85,2 %, and 48,7 % for Ig M. The Immuncapture test was found to be compatible with ELISA Ig M and Ig G tests but it was statistically incompatible with Coombs anti-brucella test. Conclusions: Immuncapture agglutination test yields similar results to those of Coombs anti-brucella test. This test is a useful test by virtue of the fact that it determines blocking antibodies in the diagnosis and follow-up of brucellosis. © Ivyspring International Publisher.
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    Investigation of microbial colonization of computer keyboards used inside and outside hospital environments [Hastane ve hastane d?ş? ortamlarda kullan?lan bilgisayarlar?n klavyelerinde mikrobiyal kolonizasyonun araşt?r?lmas?]
    (2008) Do?an M.; Feyzio?lu B.; Özdemir M.; Baysal B.
    Computers have been commonly used in daily life and at hospitals by medical staff. This study was carried out to search the microbial colonization of computer keyboards and mice used inside and outside hospital environments. Keyboards and mice samples from a total of 398 computers were included to the study, in which 38 were used by doctors and nurses in the hospital clinics (Group 1); 32 by the medical faculty students (Group 2), and 328 by university students (Group 3) in the computer laboratories of Selcuk University, Konya (located at middle Anatolia). Of the computers, 96.7% (n:385) have been found to be colonized by coagulase-negative staphylococci (CoNS), 13.1% (n:52) by gram-positive spore-forming bacilli and 8.8% (n:35) by corynebacteria; followed by Candida spp. (4.2%), gram-negative bacilli (1.7%) [Acinetobacter spp. (n:4), Pseudomonas sp. (n:1), Klebsiella sp. (n:1), E.coli (n:1)], Staphylococcus aureus (1.5%), and molds (Penicillium, Aspergillus; 1.2%). The isolation rates of CoNS were similar between the groups (94,7%, 93.7%, and 97.2%, respectively). However it was noted that all of the gram-negative bacterial isolates (7/38; 18.4%) were from the samples collected from hospital computers (Group 1). Susceptibility rates of CoNS isolates to cefoxitin were detected as 26.2% in Group 1, 79.2% in Group 2, and 91.3% in Group 3. Five out of six S.aureus strains were found susceptible to cefoxitin, except one isolated from a sample of Group 1. Linezolid resistance in both CoNS and S.aureus isolates were not determined in any groups. As a result, according to the data obtained from this study as well as from the other foreign studies, the computer keyboards and mice which are widely used in the hospital settings, are being the source of potential cross contamination in the development of nosocomial infections. Therefore the computers should be cleaned properly frequently and hand washing procedures and disinfection rules should be obeyed after the use of computers before handling this patients.
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    Investigation of viral pathogens during pregnancy in a city region in Turkey [Türki?ye'ni?n bi?r şehri?nde gebeli?k sirasinda vi?ral patojenleri?n araştirilmasi]
    (2011) Özdemir M.; Kalem F.; Feyzio?lu B.; Baysal B.
    Rubella virus, Cytomegalovirus (CMV) and Herpes simplex virus (HSV)-II are the infectious agents which may cause congenital malformations in the fetus if acquired during pregnancy. Thus these agents must be screened throughout pregnancy. This screening is also important to demonstrate the regional status regarding these agents. In this study; it is aimed to evaluate the seroprevalance of Rubella, CMV and HSV-II in pregnant women in a city region in Turkey. A total of 249 pregnant women who applied to the outpatient clinics of the obstetric and gynaecological department of a university hospital were involved to the study during antenatal screening in the first trimester of pregnancy. After separation of blood sample sera samples were tested for antibodies to Rubella, CMV and HSV-II. While Rubella and CMV antibodies were tested using Enzyme Linked Floeresan Antibody method, HSV-II was tested using ELISA. The mean age of the participants in this study was 28.4± 3.4 years. The seropositivity for anti-Rubella IgM, anti-Rubella IgG, anti-CMV IgM, anti-CMV IgG, anti-HSV-II IgM, and anti-HSV-II IgG were 0.4%, 95.9%, 0%, 98.7%, 0%, 4.4%, respectively. Although the seropositivity of HSV-II was very low, seropositivities of Rubella and CMV were very high in our area of investigation. Viruses causing congenital infection and malformation must be screened in pregnant women individually and avidity tests must be applied in suspicious cases. In addition the seroprevelance of these viral infections should be known in every population in order to take preventive measures and prepare local and general health policies.