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Öğe ABM/P-15 modulates proliferation and mRNA synthesis of growth factors of periodontal ligament cells(TAYLOR & FRANCIS LTD, 2009) Emecen, Pinar; Akman, Abdullah Cevdet; Hakki, Sema Sezgin; Hakki, Erdogan Esref; Demiralp, Burak; Toezeum, Tolga Fikret; Nohutcu, Rahime MeralObjective. Periodontal regeneration is histologically defined as regeneration of the tooth supporting structures, including alveolar bone, periodontal ligament, and cementum. Cells in the remaining periodontal tissues need optimal conditions if they are to perform their functions in the regeneration process. The present study is an investigation of the molecular effects of ABM/P-15 on human periodontal ligament cells (PDL) in vitro. Material and methods. PDL cells obtained from healthy subjects were used for in vitro experiments. Cell proliferation, morphology, and mineralization using Von kossa staining were evaluated. mRNA expressions for transforming growth factor- (TGF-), insulin-like growth factor-I (IGF-I), basic fibroblast growth factor (b-FGF), vascular endothelial growth factor (VEGF), bone morphogenic protein-2 (BMP-2), platelet-derived growth factor (PDGF), and type 1 collagen (COL1) were assessed on days 3 and 7 using RT-PCR. Results. ABM/P-15 enhanced proliferation of cultured PDL cells. It increased the mRNA expression of TGF- and BMP-2 in cultured PDL cells on days 3 and 7. IGF-I and b-FGF mRNA expressions showed a slight decrease, while PDGF expression was observed to have increased on day 3. VEGF and COL1 mRNA expressions were found not to be different on days 3 and 7. No differences were observed in the mineralization properties of cultured PDL cells treated with or without ABM/P-15. Conclusions. Based on the results of this in vitro study, it may be concluded that ABM/P-15 enhanced the regenerative capacity of PDL by regulating specific gene expressions of cells during early wound healing.Öğe Antioxidant responses of pumpkin genotypes exposed to drought stress conditions(ELSEVIER SCIENCE BV, 2018) Hamurcu, Mehmet; Avsaroglu, Zuhal Zeynep; Ustun, Canan; Omay, Humeyra; Gezgin, Sait; Hakki, Erdogan Esref; Pandey, Anamika[Abstract not Available]Öğe Biochemical effects of drought stress on two Turkish watermelon varieties are different and influenced by nitric oxide(ELSEVIER SCIENCE BV, 2016) Hamurcu, Mehmet; Hakki, Erdogan Esref; Demiral, Tijen; Gezgin, Sait[Abstract not Available]Öğe Boron deficiency responses on nutrients composition in brachypodium distachyon, a model for wheat(ELSEVIER SCIENCE BV, 2017) Pandey, Anamika; Khan, Mohd Kamran; Hamurcu, Mehmet; Gezgin, Sait; Hakki, Erdogan Esref[Abstract not Available]Öğe Chromosome numbers of species of the genus Jurinea Cass. (Asteraceae) in Turkey(UNIV FLORENCE BOTANY INST, 2009) Bekir, Dogan; Duran, Ahmet; Martin, Esra; Hakki, Erdogan EsrefChromosome numbers of 13 Jurinea species, collected from different localities in Turkey, were determined for the first time. All the Jurinea species investigated were diploid with chromosome numbers counted as 2n = 34. The basic chromosome number of the genus was x = 17. In this study, polyploidy was determined in nine taxa of Jurinea. Polyploid cells were 2n = 4x = 68 and they were tetraploid. The mitotic metaphase plates were given all the taxa of Jurinea. The research has made contribution to the cytotaxonomic revision of the genus Jurinea in Turkey.Öğe Effect of nitric oxide on the tolerance mechanism of bread wheat genotypes under salinity stress(ELSEVIER SCIENCE BV, 2014) Hakki, Erdogan Esref; Hamurcu, Mehmet; Demiral, Tijen; Avsaroglu, Zuhal Zeynep; Calik, Merve; Zengin, Mehmet; Topal, Ali[Abstract not Available]Öğe The effects of toxic level boron treatments on the bio-chemical features of Puccinellia distans(CURRENT BIOLOGY LTD, 2011) Hamurcu, Mehmet; Demiral, Tijen; Gezgin, Sait; Caglar, Hayriye; Kizmaz, Veysi; Hakki, Erdogan Esref[Abstract not Available]Öğe Evaluation of puroindoline b as a house keeping gene in wild wheat species(CURRENT BIOLOGY LTD, 2011) Hakki, Erdogan Esref; Uygan, Songul; Hamurcu, Mehmet; Gezgin, Sait; Bozkurt, Buket Serife; Kayis, Seyit Ali[Abstract not Available]Öğe Evaluation of serine carboxypeptidase (PsCP) gene expression in Pisum arvense L. grown under different boron levels(ELSEVIER SCIENCE BV, 2012) Hakki, Erdogan Esref; Tamkoc, Ahmet; Hamurcu, Mehmet; Can, Nimet; Demiral, Tijen; Gezgin, Sait; Ozdemir, Canan[Abstract not Available]Öğe Implication of nitric oxide on growth and development of wheat under drought conditions(ELSEVIER SCIENCE BV, 2014) Demiral, Tijen; Hamurcu, Mehmet; Avsaroglu, Zuhal Zeynep; Calik, Merve; Almas, Semra; Hakki, Erdogan Esref; Topal, Ali[Abstract not Available]Öğe Issr Analysis of Perennial Ryegrass (Lolium Perenne L.) Genotypes and Association Study Between Phenotypes and Genotypes(Elsevier Science Bv, 2012) Kayis, Seyit Ali; Tamkoc, Ahmet; Uygan, Songul; Hakki, Erdogan EsrefBreeders can efficiently use this molecular information in their breeding programs by adding and removing photoperiod insensitive Ppd-D1a alleles to their varieties. In hexaploid wheat, photoperiod insensitive PpdD1a allele in 2D chromosome causes early flowering in short day length and long day length, avoiding stresses associated with high temperature and water deficit in grain filling stages. This study shows that the parents NE01533, Pelsart, Promontory and the 47 lines in F2 generation have the Ppd-D1a allele while Yellowstone and the remaining lines are photoperiod sensitive.Öğe Oxidative stress responses in watermelon (Citrullus lanatus) as influenced by boron toxicity and drought(LITHUANIAN RESEARCH CENTRE AGRICULTURE & FORESTRY, 2015) Hamurcu, Mehmet; Demiral, Tijen; Hakki, Erdogan Esref; Turkmen, Onder; Gezgin, Sait; Bell, Richard W.This study aimed to investigate the effect of different boron (B) treatments on drought tolerance of watermelon plants. Drought tolerant Citrullus lanatus (Thunb.) Matsum. et Nakai genotype 'Kar 98' was grown in controlled greenhouse conditions hydroponically and exposed to drought stress by applying PEG 6000 (polyethylene glycol) in the presence of three boron dosages: 0.05, 0.25 and 1.25 mM. Growth parameters (fresh weight, dry weight and lengths of shoot and roots), leaf relative water content, boron accumulation, lipid peroxidation level and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) enzymes were determined as well as the accumulation of hydrogen peroxide (H2O2) and hydroxyl (center dot OH)-scavenging activity were assayed. Increasing dosages of boron alone caused more severe growth reduction than combined with PEG 6000-induced drought stress. Induced drought stress caused less accumulation of boron in leaves and roots. B concentration of 1.25 mM caused lipid peroxidation in a reactive oxygen species-independent manner and drought stress-induced lipid peroxidation was alleviated by increasing B dosages. Induced glutathione reductase activity under the combination of 1.25 mM B and PEG 6000-induced drought stress seemed an important physiological response in 'Kar 98' plants against multiple stresses.Öğe Physiological responses of two contrasting watermelon genotypes exposed to drought and nitric oxide(ELSEVIER SCIENCE BV, 2016) Hakki, Erdogan Esref; Hamurcu, Mehmet; Gezgin, Sait; Demiral, Tijen[Abstract not Available]Öğe SDF-1 modulates periodontal ligament-Mesenchymal Stem Cells (pdl-MSCs)(ELSEVIER SCIENCE BV, 2017) Hakki, Sema Sezgin; Bozkurt, Serife Buket; Hakki, Erdogan Esref; Karaoz, Erdal; Unlu, Ali; Kayis, Seyit Ali[Abstract not Available]
