Yazar "Ozkalp, Birol" seçeneğine göre listele
Listeleniyor 1 - 9 / 9
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Antimicrobial activities of some mushrooms in Turkey(TAYLOR & FRANCIS LTD, 2013) Dogan, Hasan Huseyin; Duman, Rustem; Ozkalp, Birol; Aydin, SemaContext: Terfezia boudieri Chatin (Pezizaceae), Agaricus brunnescens Peck (Agaricaceae) and Lactarius vellereus (Fr.) Fr. (Russulaceae) are well-known species in Turkey, and are used both for food and traditional medicine. Objective: The powdered fruit bodies of T. boudieri, A. brunnescens and L. vellereus were used to evaluate the antimicrobial activities. Materials and methods: Chloroform, acetone and methanol extracts of T. boudieri, A. brunnescens and L. vellereus were tested for their antimicrobial activities against four Gram-positive bacteria, five Gram-negative bacteria and yeast using a micro-dilution method. Results: The strongest minimum inhibitory concentration (MIC) value observed against the test microorganisms was with the chloroform extract of T. boudieri (MIC 2.4 mu g/mL) against Streptococcus pyogenes. Maximum antimicrobial effects were observed with the acetone extracts of T. boudieri and L. vellereus (MIC 4.8 mu g/mL) against Bacillus subtilis. The strongest antifungal activity was observed with the acetone extracts of T. boudieri (MIC 2.4 mu g/mL) and A. brunnescens (MIC 19.5 mu g/mL) against Candida albicans. The strongest MIC values for all fungal extracts were observed between 78 and 2.4 mu g/mL. Discussion and conclusion: Present results demonstrated that these three mushroom species have excellent antimicrobial and antifungal activities, and thus have great potential as a source for natural health products.Öğe Distribution of ıntestinal parasites for age and gender in the 13 to 18 years age group at the Nigde Orphanage(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2010) Ozkalp, Birol; Celik, Battal; Kurtoglu, Muhammet Guzel; Kesli, RecepThe present study was aimed at the determination of the rate of infection with intestinal parasites in children cared for at the Nigde Orphanage, and the demonstration of the distribution of infection in these children for age and gender. Faecal samples collected from 110 children, aged 13 to 18 years, were examined by the native method using physiological saline. Of the 110 faecal samples examined, 41 (37.3%) contained intestinal parasites, and of the samples containing parasites, 25 (61%) contained helminths and 16 (39%) contained protozoa. The number of faecal samples identified to contain a single parasite species was 39 (95%), while 2 (5%) samples contained more than one parasite species. As regards the distribution of the intestinal parasites for gender, it was ascertained that of the 75 females 30 (40%) individuals and of the 35 males 11 (31.5%) individuals were infected with intestinal parasites. The difference between the two sexes was considered to be insignificant. Faecal examination revealed the presence of the following parasite species at the indicated rates: Ascaris lumbricoides (34.1%), Entamoeba histolytica (29.3%), Taenia saginata (17.1%), Giardia intestinalis (9.8%), Enterobius vermicularis (7.3%) Trichuris trichiura (2.4%). The present study demonstrated that the environment of the orphanage was favourable for autoinfection and the spread of infection by direct contact. Furthermore, it was determined that the rate of parasitic infection was higher in the age group of 13 to 14 years, compared to the age group of 15 to 18 years.Öğe Effect of sildenafil (viagra (r)) on penile erection and semen volume and characteristics in kangal dogs(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2009) Coyan, Kenan; Ataman, M. Bozkurt; Ozkalp, Birol; Tepeli, CaferIn this study, the effects of sildenafil administration on penile erection and semen volume and characteristics in Kangal dogs were studied. A total of 5 Kangal dogs, aging 3-6 years, and with a known fertility were used. In each application, sildenafil was administered orally at a dosage of 50 mg, 60 minutes prior to semen collection. Semen was collected 12 times at 3-day intervals by digital manipulation. After collected, semen was examined for volume (first, second, and third fractions), ejaculation time, and spermatological characteristics. Dogs were rested for 3 weeks after the last semen collection. Following the rest period, semen collection was experienced 12 times at 3-day intervals by digital manipulation without sildenafil application. Sildenafil administration enhanced the volume of the second and third fractions, compared to the non-sildenafil group (1.1 ml versus 0.7 ml and 11 ml versus 6 ml, respectively, P<0.05), and increased the total ejaculation time (11.8 minutes versus 7 minutes, P<0.05). On the other hand, there were no significant differences between the sildenafil and non-sildenafil groups for spermatological characteristics (P>0.05). As a conclusion, sildenafil could be used in dogs, having problems in response to digital manipulation, for penile erection and successful semen collection.Öğe The effect of simulator-education on students receiving education at the department of elderly care(ELSEVIER SCIENCE BV, 2015) Saygili, Ulku; Ozkalp, BirolUsage and development of new learning tools increased along with the developing technology in health education. The changing expectations in the health services drew attention to clinical skill trainings. Patient safety, which is among the rising values, the struggle to increase the patient rights and student competency, enabled the simulation usage getting more and more widespread in the health education. These developments rising from the technology and education paved the way for the applications and tools of simulation, which is used widespread in enhancing the technical and non-technical skills in health education and which is among the trust worthy education methods, by bringing these two fields together along with them. Using simulation helps students have increased self-esteem and develop their ability to make clinical decisions by providing learning based on experience. Because it contributes to the experiences and occupational skills of the students positively at the same time, the goal of increasing the clinical competence of the students in a virtual or laboratory environment prior to the patient care environment is accomplished. Before going out to the clinic, students can develop their occupational skills in a laboratory that reflects a real clinical environment that is integrated with the life like scenario. (C) 2015 The Authors. Published by Elsevier Ltd.Öğe The effectiveness of similitor usage in the paramedic education(ELSEVIER SCIENCE BV, 2015) Ozkalp, Birol; Saygili, UlkuTurkey, the educational simulation has an important place in terms of this changing sense in medical education. The universities, which review the training they provide, involve the simulation in their curriculum in order to enable the students-to-graduate to acquire the need of convenient knowledge and some skills during the faculty education. The team that is employed in 112 emergency health services primarily aims to perform interventions for saving the human life in cases of any danger, try to prevent the possible secondary damages while performing these interventions and thus, enable the sustainment of life without any sequela. Simulation presents a learning environment that provides the possibility of a learner-centered experience rather than an experience where the patient is objective, and gives both confidence and support to the student. (C) 2015 Published by Elsevier Ltd.Öğe Effects of hypotaurine, cysteamine and aminoacids solution on post-thaw microscopic and oxidative stress parameters of Angora goat semen(ELSEVIER SCI LTD, 2009) Bucak, Mustafa Numan; Tuncer, Puerhan Barbaros; Sariozkan, Serpil; Ulutas, Pinar Alkim; Coyan, Kenan; Baspinar, Nuri; Ozkalp, BirolThis study was conducted to determine the effects of cysteamine, hypotaurine and aminoacids solution (BME) on standard semen parameters, lipid peroxidation and antioxidant activities of Angora goat semen after the freeze-thawing process. Ejaculates collected from four Angora goats were evaluated and pooled at 37 degrees C. Semen samples, which were diluted with a Tris-based extender containing the antioxidants hypotaurine (5 mM) and cysteamine (5 mM), and an aminoacid solution (13%), and an extender containing no antioxidants (control), were cooled to 5 degrees C and frozen in 0.25-ml French straws in liquid nitrogen. Frozen straws were thawed individually at 37 degrees C for 20 s in a water bath for evaluation. Supplementation with cysteamine, hypotaurine and BME caused significant (P < 0.05) increases in sperm motility, and significant (P < 0.05) decreases in total abnormality rates in comparison to the control group. While all in vitro treatments did not affect the acrosomal abnormality rates, hypotaurine and BME but not cysteamine significantly (P < 0.05) increased the HOST results as compared to the control group. Supplementation with antioxidants and BME did not significantly affect MDA levels and CAT activity in comparison to the control group (P > 0.05). The antioxidants hypotaurine and cysteamine decreased SOD activity when compared to the BME group and controls (P < 0.001). (c) 2009 Elsevier Ltd. All rights reserved.Öğe Isolation and Identification of Salmonellas from Different Samples(INTECH EUROPE, 2011) Ozkalp, Birol[Abstract not Available]Öğe Isolation Rate of Mycobacterium tuberculosis Complex from Patients with Suspected Tuberculosis and Identification of the Strains with BACTEC (TM) NAP and Immunochromatographic TB Ag MPT64 Rapid (TM) Tests(ANKARA MICROBIOLOGY SOC, 2011) Kurtoglu, Muhammet Guzel; Ozdemir, Mehmet; Kesli, Recep; Ozkalp, Birol; Baysal, BulentTuberculosis (TB) which is still one of the important infectious diseases in the world as well as Turkey, results in high morbidity and mortality. Clinical mycobacteriology laboratories have crucial roles in the identification, typing and susceptibility testing of Mycobacterium tuberculosis. The aims of this study were the investigation of the isolation rate of M.tuberculosis complex (MTC) from the clinical specimens of TB-suspected patients and to compare identification of mycobacteria isolated from solid and/or liquid media by using BACTEC NAP and immunochromatographic TB Ag MPT64 rapid test. A total of 1670 patients who were admitted to outpatients clinics of our hospital and prediagnosed as TB, have been included in the study. All the patients were anti-HIV seronegative. NALC-NaOH method were used for decontamination/homogenization, and preparations from samples were stained with Erlich-Ziehl-Neelsen method to detect acid-resistant bacilli (ARB) in direct microscopy. All of the samples were inoculated into BACTEC (TM) MGIT-960 (Becton Dickinson, USA) and Lowenstein-Jensen (14) media for cultivation and incubated at 37 degrees C for 6-8 weeks. Mycobacteria that were grown in the media have been identified by BACTEC (TM) NAP (Becton Dickinson, USA) and TB Ag MPT64 rapid test (SD Bioline Ag MPT64 Rapid (TM); Standard Diagnostics, Korea). The culture positivity in the samples of TB-suspected patients was found to be 3.7% (63/1670) with LJ and/or MGIT-960 methods, whereas ARB positivity rate was 1.6% (28/1670). Fifty-three (84%) out of culture positive 63 samples have been identified as MTC by BACTEC NAP test, while 61(97%) were found as MTC by TB MPT64 test. Considering BACTEC NAP test as the reference method, TB MPT64 test identified all the MTC strains correctly (sensitivity: 100%), however the false positivity rate was estimated as 12.7% (specificity: 87%). Of 53 MTC positive samples, 36 were sputum, four were bronchoalveolar lavage, four were urine, three were gastric fluid, three were pleural fluid, and one of each were abscess, peritoneal fluid and cerebrospinal fluid samples. ARB positivity rate was detected as 41.5% (22/53) among MTC culture positive samples. Of the patients who were infected with MTC, 72% (38/53) were male and 98% (52/53) were adults (age range: 20-85 years). Our data indicating 3.1% (53/1670) isolation rate of MTC from TB-suspected patients in our region were in concordance with the other results reported from Turkey. In conclusion, immunochromatographic TB Ag MPT64 test which seemed to be useful for the rapid identification of mycobacteria grown on solid and/or liquid, was practical to perform and had high sensitivity, however further larger-scaled studies are needed to support our data in our country.Öğe Synthesis, spectroscopic characterization and antimicrobial studies of Co(II), Ni(II), Cu(II) and Zn(II) complexes with Schiff bases derived from 5-bromo-salicylaldehyde(ELSEVIER SCIENCE BV, 2013) Kursunlu, Ahmed Nuri; Guler, Ersin; Sevgi, Fatih; Ozkalp, BirolIn this study, the new Schiff base ligands derived from condensation of amine and 5-bromo-salicylaldehyde were characterized. All compounds, the Schiff bases and the metal complexes, were characterized by elemental analyzes, FT-IR, H-1 NMR, C-13 NMR and magnetic susceptibility measurements. The synthesized ligands, along with their metal (II) complexes, were screened for their in vitro antibacterial activity against four Gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella enteritidis) and four Gram-positive (Streptococcus pyogones, Bacillus cereus, Staphylococcus aureus and Methicillin-resistant S. aureus) bacterial strains by using disc diffusion and broth microdilution techniques. The minimum inhibitory concentration (MIC) values were calculated by microplate reader at 620 nm to. optimum result. It was found that all compounds showed mild to moderate antibacterial activity and the Schiff base ligands had a better antibacterial activity than their complexes. (c) 2013 Published by Elsevier B.V.
