Polyvinylferrocenium modified Pt electrode for anaerobic glucose monitoring
Yükleniyor...
Tarih
2005
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
ELSEVIER SCIENCE BV
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
An amperometric enzyme electrode for the determination of glucose under anaerobic solution conditions was developed by immobilizing glucose oxidase and then by adsorbing ferrocene in polyvinylferrocenium matrix coated on a Pt electrode surface. The amperometric response due to the electrooxidation of ferrocene that the reduced flavin adenine dinucleotide centers of glucose oxidase was measured at a constant potential. The response characteristics of the enzyme electrode were investigated. The effects of the thickness of the polymeric film, the amount of the enzyme immobilized, the amount of the mediator, the glucose concentration, the applied potential, operating pH and temperature on the response of the enzyme electrode were studied. The response time and the optimum pH were found to be 30-40 s and pH 7.4 at 25 T, respectively. The linear response was observed up to 5.0 mM glucose concentration that the produced detectable current was 0.0075 W glucose concentration. The activation energy (E.) of immobilized enzyme reaction was calculated to be 41.3 kJ mol(-1) from the Arrhenius plot. The apparent Michaelis-Menten constant (K-Mapp) was found to be 6.05 mM glucose according to the Lineweaver-Burk graph of the Michaelis-Menten equation under the optimum conditions. The interference signal due to the most common electrochemical interfering species was also evaluated. (C) 2004 Elsevier B.V All fights reserved.
Açıklama
Anahtar Kelimeler
polyvinylferrocenium, ferrocene, glucose, enzyme electrode
Kaynak
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
62
Sayı
1
