Retained acetylated histone four in bull sperm associated with fertility

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dc.contributor.authorUgur, Muhammet Rasit
dc.contributor.authorKutchy, Naseer Ahmad
dc.contributor.authorde Menezes, Erika Bezerra
dc.contributor.authorUl-Husna, Asma
dc.contributor.authorHaynes, Bethany Peyton
dc.contributor.authorUzun, Alper
dc.contributor.authorKaya, Abdullah
dc.contributor.authorTopper, Einko
dc.contributor.authorMoura, Arlindo
dc.contributor.authorMemili, Erdogan
dc.date.accessioned2020-03-26T20:19:14Z
dc.date.available2020-03-26T20:19:14Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Veteriner Fakültesi, Klinik Bilimler Bölümüen_US
dc.description.abstractBull fertility, ability of the sperm to fertilize and activate the egg and support embryo development, is vital for cattle reproduction and production. Even though majority of histories are replaced by protamines, some histories are retained in sperm. It is known that chromatin remodeling during spermatogenesis results in dynamic changes in sperm chromatin structure through post-translational modifications (PTM) of sperm histones, which are important for regulation of gene expression. However, amounts of sperm Histone 4 (H4), its acetylated form (H4 acetyl), and to what extent these molecular attributes influence sperm chromatin structure and bull fertility are unknown. These gaps in the knowledge base are important because they are preventing advances in the fundamental science of bovine male gamete and improvement of bull fertility. The objective of this study was to test the hypothesis that expression dynamics as well as PTM of sperm H4 are associated with bull fertility. Flow cytometry was utilized to quantify H4 and H4 acetylated form in sperm from seven high and seven low fertility Holstein bulls. The results indicated that the average number of cells with H4 or H4 acetyl expression in high and low fertility bull sperm were 34.6 +/- 20.4, 1.88 +/- 1.8, 15.2 +/- 20.8, and 1.4 +/- 1.2, respectively. However, the sperm enriched in both H4 and H4 acetyl were different between high and low fertility groups (3.5 +/- 0.6; 1.8 +/- 0.8; P = 0.043). The localization and detection of H4 and H4 acetylation were measured by immunocytochemistry which revealed that H4 and H4 acetylation were equally distributed in the sperm head of high and low fertility sires. Western blotting results confirmed the presence of the H4 and its acetylated form in the sperm. Bioinformatics studies demonstrated that H4 is highly conserved among mammalians, and have significant gene ontology on spermatogenesis, early embryo implantation, and sperm capacitation. The results are significant because it demonstrates the replacement of canonical histone H4 into modified H4 acetylation in sperm and regulate its dynamics which is crucial for bull fertility and reproductive biotechnology. These findings advance fundamental science of mammalian early development and reproductive biotechnology.en_US
dc.description.sponsorshipMississippi Agricultural Forestry Experiment Station; Alta Genetics Inc., Wisconsin, USA; Coordination for the improvement of higher education personnel (CAPES) from BrazilCAPES; Netaji-Subhas Indian Council of Agricultural Research (ICAR)Indian Council of Agricultural Research (ICAR); Turkish Ministry of National EducationMinistry of National Education - Turkeyen_US
dc.description.sponsorshipStudy funded in part by Mississippi Agricultural Forestry Experiment Station, and by Alta Genetics Inc., Wisconsin, USA. EBM was funded through a competitive fellowship from the Coordination for the improvement of higher education personnel (CAPES) from Brazil. NK was funded through a competitive fellowship from Netaji-Subhas Indian Council of Agricultural Research (ICAR). MU was funded through competitive graduate fellowship from the Turkish Ministry of National Education. Some of the results from this study are presented at the SSR conference by Ugur et al. (14).en_US
dc.identifier.citationUgur, M. R., Kutchy, N. A., Menezes, E. B. D., Ul-Husna, A., Haynes, B. P., Uzun, A., Kaya, A., Topper, E., Moura, A., Memili, E. (2019). Retained Acetylated Histone Four İn Bull Sperm Associated With Fertility. Frontiers İn Veterinary Science, 6, 223.
dc.identifier.doi10.3389/fvets.2019.00223en_US
dc.identifier.issn2297-1769en_US
dc.identifier.pmid31417913en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://dx.doi.org/10.3389/fvets.2019.00223
dc.identifier.urihttps://hdl.handle.net/20.500.12395/38168
dc.identifier.volume6en_US
dc.identifier.wosWOS:000477988500001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaya, Abdullah
dc.language.isoenen_US
dc.publisherFRONTIERS MEDIA SAen_US
dc.relation.ispartofFRONTIERS IN VETERINARY SCIENCEen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectacetylationen_US
dc.subjectepigeneticsen_US
dc.subjecthistone 4en_US
dc.subjectfertilityen_US
dc.subjectspermen_US
dc.titleRetained acetylated histone four in bull sperm associated with fertilityen_US
dc.typeArticleen_US

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