In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium

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dc.contributor.authorEruygur, Nuraniye
dc.contributor.authorUçar, Esra
dc.contributor.authorAkpulat, Hüseyin Aşkın
dc.contributor.authorShahsavari, Keyhan
dc.contributor.authorSafavi, Seyed Mehdi
dc.contributor.authorKahrizi, Danial
dc.date.accessioned2020-03-26T20:14:40Z
dc.date.available2020-03-26T20:14:40Z
dc.date.issued2019
dc.departmentSelçuk Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümüen_US
dc.description.abstractHypericum lydium Boiss. is a perennial plant of the Hypericaceae family, which has been used in particular to treat depression. The aim of this study was to determine in vitro antioxidant, antimicrobial activities, anticholinesterase (acetylcholinesterase (AChE)/butyrylcholinesterase (BChE)), antidiabetic activities (-glucosidase/-amylase) and Tyrosinase inhibitor activity of methanol and water extracts of H. lydium. Also, gene expression has been evaluated in the shoot and root by microarray technology. So, in general, the purpose of this study is to study the active molecules such as antioxidant, antimicrobial, antidiabetic, enzymes and genes in the plant, which is the first to be reported. The experiments were conducted in a completely randomized design with three replications. In addition, gene expression was compared in the shoot and root parts. Expression profiling was carried out by microarrays. According to the results, the highest chemical components were determined in methanol extract rather than water extract. There was a difference between the obtained components. While the highest antioxidant activity was determined from the methanol extract of plant herbs for DPPH Free Radical Scavenging Activity, antioxidant activity was the same in both methanol and water extracts using the ABTS method. The methanol extract demonstrated stronger anticholinesterase (AChE and BChE) and -amylase inhibition activity. This study was complemented by the detection of antioxidant activity and some enzyme inhibition activity in the methanol extract. Microarray showed 10,784 genes had significantly different expression in root and shoot. There was a positive effect of methanol extract in respect of different activities compared to the water extract. Gene expression showed that the number of expressed genes in the root was greater than the shoot.en_US
dc.identifier.citationEruygur, N., Ucar, E., Akpulat, H. A., Shahsavari, K., Safavi, S. M., Kahrizi, D. (2019). In Vitro Antioxidant Assessment, Screening Of Enzyme İnhibitory Activities Of Methanol And Water Extracts And Gene Expression İn Hypericum Lydium. Molecular Biology Reports, 46,(2), 2121-2129.
dc.identifier.doi10.1007/s11033-019-04664-3en_US
dc.identifier.endpage2129en_US
dc.identifier.issn0301-4851en_US
dc.identifier.issn1573-4978en_US
dc.identifier.issue2en_US
dc.identifier.pmid30762165en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2121en_US
dc.identifier.urihttps://dx.doi.org/10.1007/s11033-019-04664-3
dc.identifier.urihttps://hdl.handle.net/20.500.12395/37915
dc.identifier.volume46en_US
dc.identifier.wosWOS:000463996700055en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorEruygur, Nuraniye
dc.language.isoenen_US
dc.publisherSPRINGERen_US
dc.relation.ispartofMOLECULAR BIOLOGY REPORTSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.selcuk20240510_oaigen_US
dc.subjectHypericum lydiumen_US
dc.subjectGene expressionen_US
dc.subjectAnticholinesteraseen_US
dc.subjectAntimicrobial and antidiabetic activityen_US
dc.titleIn vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydiumen_US
dc.typeArticleen_US

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