Genetic characterization of Turkish cattle breeds by microsatellite markers: Usefulness for parentage testing
Yükleniyor...
Dosyalar
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
KAFKAS UNIV, VETERINER FAKULTESI DERGISI
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Objective of this study was to evaluate microsatellite markers in paternity testing of native cattle breeds in Turkey. Blood samples were collected from Anatolian Black (n=51), Anatolian Grey (n=54), South Anatolian Red (n=51), Native Southern Anatolian Yellow (n=51), East Anatolian Red (n=45) and Zavot (n=19) cattle. From the blood samples DNA was isolated by using a standard phenol/chloroform method. A total of 20 microsatellite loci were selected from a FAO/ISAG-suggested list. Polymerase chain reaction products were separated by capillary electrophoresis and marker genotypes were determined by fragment analysis. In statistical analyses, allel numbers, observed (Ho) and expected (He) heterozygosities, deviation from Hardy-Weinberg Equilibrium and probability of exclusion (PE) at each microsatellite locus were calculated. A total of 269 different alleles were observed and the mean allele was identified as 13.45. Mean Ho and He values were observed as 0.619-0.852 and 0.669-0.877, respectively. The results indicated that the microsatellite test panel including the most informative 7 loci had total PE value of >0.9999 in each populations and can thereby be used for parentage testing studies of native cattle breeds in Turkey.
Açıklama
Anahtar Kelimeler
Cattle, Microsatellite, Parentage testing, TURKHAYGEN-I
Kaynak
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
20
Sayı
4
Künye
Özşensoy, Y., Kurar, E., Doğan, M., Bulut, Z., Nizamlıoğlu, M., Işık, A., Çamlıdağ, A., Altunok, V. (2014). Genetic Characterization of Turkish Cattle Breeds by Microsatellite Markers: Usefulness for Parentage Testing. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20(4), 521-526.