Yıldırım, Mahmut SelmanÖztürk, KayhanAcar, HasanArbağ, HamdiÜlkü, Çağatay Han2020-03-262020-03-262003Yıldırım, M. S., Öztürk, K., Acar, H., Arbağ, H., Ülkü, C. H., (2003). Chromosome 8 Aneuploidy in Acquired Cholesteatoma. Acta Oto-Laryngologica, 123(3), 372-376. Doi: 10.1080/000164803100012860001-6489https://dx.doi.org/10.1080/00016480310001286https://hdl.handle.net/20.500.12395/18392Objective-To investigate the incidence of chromosome 8 aneuploidy in acquired cholesteatoma. Material and methods-Cholesteatoma tissue and postauricular skin as a control were surgically obtained from 12 patients with acquired cholesteatoma. Fluorescence in situ hybridization (FISH) analysis using a chromosome 8-specific alpha-satellite DNA probe was performed on the interphase nuclei. Two hundred cells were analyzed for each of the samples. Results-Chromosome 8 aneuploidy was found in 9/12 patients whereas a normal cell structure with 2 signals was observed in the remaining 3 patients. In samples with chromosome 8 aneuploidy, the mean proportion of aneuploidy was 25.6%, including monosomy (3.2%), trisomy (16.1%), tetrasomy (4.9%) and more than tetrasomy (1.4%). The number of aneuploid cells in recurrent cases was more than that in non-recurrent cases. Conclusions-A numerical aberration of chromosome 8 was found in patients with acquired cholesteatoma. Our results support the hypothesis that chromosome 8 may be a prognostic factor for cholesteatoma and an indicator in the follow-up of patients with cholesteatoma.en10.1080/00016480310001286info:eu-repo/semantics/openAccessCholesteatomaChromosome 8Fluorescence in Situ HybridizationLocalizationRecurrenceArticle123337237612737293Q2WOS:000182665700007Q2