Atalık, Kısmet Esra NurullahoğluNurullahoğlu, Zahide ÜlyaKılıç, M.2020-03-262020-03-2620110031-6903https://dx.doi.org/10.1248/yakushi.131.661https://hdl.handle.net/20.500.12395/26791In the present study, the effects of cooling (to 28 degrees C) on the vasodilatation induced by diazoxide (10(-9)-3x 10(-4) M), isoproterenol (10(-9)-3x 10(-4) M) and magnesium sulphate (0.1-30 mM) on serotonin-pre-contracted human umbilical artery and the role of nitric oxide in these effects were analyzed. Diazoxide, isoproterenol and magnesium produced concentration-dependent relaxation of human umbilical artery precontracted with serotonin (10(-6) M). During cooling, the pIC(50) values and maximal responses to these agents were significantly lower than at 37 degrees C. Cooling to 28 degrees C in the presence of N-G-nitro-L-arginine methyl ester (L-NAME, 10(-4) M) did not modify the effects of temperature on diazoxide, isoproterenol and magnesium-induced relaxations. These results suggest that cooling-induced changes of diazoxide, isoproterenol, and magnesium sulphate in human umbilical artery are independent of nitric oxide.en10.1248/yakushi.131.661info:eu-repo/semantics/openAccesscoolingdiazoxideisoproterenolmagnesiumnitric oxideumbilical arteryArticle131566166721532262Q3WOS:000290188300001Q4