Yildirim, S.Yapar, M.Sermet, U.Sener, K.Kubar, A.2020-03-262020-03-2620081079-21041528-395Xhttps://dx.doi.org/10.1016/j.tripleo.2007.06.026https://hdl.handle.net/20.500.12395/22743Objective. To address the question whether dental pulp cells of exfoliating human deciduous teeth have some roles for controlling or regulating the root resorption via secreting key molecules (OPG, RANKL, CSF-1, TGF beta, MCP-1 and Cbfa-1) in osteoclastogenesis, we used a sensitive reverse transcriptase polymerase chain reaction (RT-PCR) method for detection of mRNA expressions for the cytokines listed. Study design. The dental pulps were retrieved from incisor and molar teeth in the late stage of shedding (n = 30) and from sound premolar teeth extracted for orthodontic reasons (control group; n = 30). The RT-PCR assays were used to identify targeted gene expression. Results. Of the cytokines examined, RANKL and CSF-1 expressions showed significantly higher occurrence in deciduous dental pulps than in permanent teeth pulpal tissues (P < .040). Conclusions. The findings may suggest an interactive role for pulp tissue cells in the physiologic root resorption process. The cells of dental pulp may have some cytokine-producing cells which mediate monocyte-macrophage lineage to form osteo/odontoclasts, and the RANKL/RANK system might be involved in human deciduous teeth resorption.en10.1016/j.tripleo.2007.06.026info:eu-repo/semantics/closedAccessArticle105111312017942342N/AWOS:000252197200020Q2