Başpınar, NBaş, A. LHaliloğlu, SElmas, MYazar, E2020-03-262020-03-2619980035-1555https://hdl.handle.net/20.500.12395/16807In this study, the effects of extracellular vitamin C on PMNL functions (phagocytic and microbicidal activity) and intracellular vitamin C levels were investigated. Neutrophils were obtained from eleven healthy Holstein heifers. Phagocytic and microbicidal activity of neutrophils were measured by fluorescent microscopic technique and intracellular vitamin C levels were determined with spectrophotometer. After first incubation (135 min., 37 degrees C) 149.76 %, 289.78 % and 462.76 % vitamin C increases were observed in PMNL, at 50, 150, 300 mu M/L of vitamin C, added to incubation media respectively. All the results compared to the controls, phagocytosis and microbicidal activity of PMNL increased by different vitamin C concentrations. A definite enhancement in PMNL functions observed in this study may have attributed to vitamin C supplemantation.eninfo:eu-repo/semantics/closedAccessvitamin Cleucocyte functionsbovineArticle14910931+Q3WOS:000077393300004Q3