Ertuğrul, KuddisiArslan, EmineTugay, Osman2020-03-262020-03-262010Ertuğrul, K., Arslan, E., Tugay, O., (2010). Characterization of Consolida Sf Gray (Ranunculaceae) Taxa in Turkey by Seed Storage Protein Electrophoresis. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi, 35(2), 97-102.0250-46851303-829Xhttps://hdl.handle.net/20.500.12395/24686Objective: This research aimed to investigate the genetic relations of Consolida S. F. Gray species collected in Turkey using seed storage proteins. This study has become significant as the taxonomic status of genus Consolida changes often. Methods: Mature seeds of 22 Consolida species were collected. Total protein were isolated from seeds collected for each species. Sodium Dodecyl Sulphate-Polycarylamide Gel Electrophoresis was performed by a standard method on a vertical slab gel. After electrophoresis, the protein bands were visualized by staining with Coomassie Brillant Blue G-250.The polymorphic bands were scored visually as present (1) or absent (0). Genetic similarity among taxa was estimated basing on Nei's homology. Results: In all, 74 polypeptide bands of different sizes ranging from 25 to 140 kDa were observed in the 22 taxa of the genus Consolida. Cluster analysis was performed using the unweighted pair-group method and arithmetic averages in Bio1D++ computer program. According to similarity coefficients, the genus Consolida was separated into the main two groups. It was discovered that the first group consisted of the species of Consolida with 5-lobed corolla, and in the second main group were the species with 3-lobed corolla. Conclusion: It is concluded that seed storage protein profiles could be useful markers in the studies of genetic relations of Consolida species. The grouping as a result of the dendogram has also supported the relationship in Flora of Turkey.eninfo:eu-repo/semantics/closedAccessConsolidaSds-pageGenetic relationTotal proteinsArticle35297102WOS:000278764100004Q4