Vural Cingilli, Hasibe2020-03-262020-03-262012Cingilli Vural, H., (2012). PCR-SSCP-DNA Sequencing Method in Detecting PTEN Gene Mutation and Its Significance in Human Breast Cancer in Turkish Populations. Biotechnology & Biotechnological Equipment, 26(5), 3220-3223. DOI: 10.5504/BBEQ.2012.00841310-2818https://dx.doi.org/10.5504/BBEQ.2012.0084https://hdl.handle.net/20.500.12395/28286The aim of this study was to analyze the possible effect of PTEN gene mutations on the occurrence and development of human breast cancer. PCR-SSCP-DNA sequencing indicated that two kinds of mutation sites were found in 4 out of 80 breast cancer samples. One kind of mutation was found in exons. AA-TCC mutation was located 40 bp upstream of the 3'lateral exon 2 (115946 AA-TCC). Such mutations led to terminator formation at codon 267 of the PTEN gene. The other mutation was found in an intron, including a C-T point mutation 91 bp upstream of 2'lateral exon 2 (1903858 C-T). The PTEN protein expression in tissues with breast cancer indicated that the total positive rate of PTEN protein expression was 68 % in breast cancer tissue, which was significantly lower than that (100 %) in paracancerous tissues (P < 0.005). PTEN gene mutation may play an important role in the occurrence and development of breast cancer.en10.5504/BBEQ.2012.0084info:eu-repo/semantics/openAccessbreast cancerPTEN genePCR-SSCPmutationArticle26532203223Q3WOS:000310398800005Q4