Ülger, HarunKarabulut, Ahmet KağanPratten, M.K.2020-03-262020-03-262002Ülger, H., Karabulut, A. K., Pratten, M. K., (2002). Labelling of Rat Endothelial Cells With Antibodies to vWF, RECA-1, PECAM-1, ICAM-1, OX-43 and ZO-1. Anatomia, Histologia, Embryologia, (31), 31-35. Doi: 10.1046/j.1439-0264.2002.00357.x0340-2096https://dx.doi.org/10.1046/j.1439-0264.2002.00357.xhttps://hdl.handle.net/20.500.12395/18158Labelling with endothelium specific monoclonal antibodies, von Willebrand Factor (vWF), rat endothelial cell antigen-1 (RECA-1), platelet-endothelial cell adhesion molecule-1 (PECAM-1), intercellular adhesion molecule-1 (ICAM-1), OX-43 and zonula occludentes-1 (ZO-1), was investigated in cryostat sections of vessels from rats of different ages using a confocal microscope. The results showed that labelling of the vWF was positive in endothelial cells from adult, fetal and different ages of embryonic rat. Labelling with RECA-1 was weakly positive in adult rat aorta and lung endothelial cells but not in embryonic yolk sac endothelial cells. Labelling using PECAM-1, ICAM-1 and OX-43 was negative in both adult and embryonic endothelial cells. ZO-1 showed positive but very weak reactivity in embryonic yolk sac endothelial cells. The expression of vWF on vessels from adult and 19.5-day fetal tissues was strongly positive. However, the expression of vWF in embryonic endothelial cells was dependent on the gestational age. While the 11.5-day yolk sac vessels stained weakly, staining gradually increased in 13.5-, 15.5- and 17.5-day-old yolk sac vessels. The results suggest that vWF is a reliable endothelial cell marker in rat vascular endothelial cells, including both fetal and embryonic stages.en10.1046/j.1439-0264.2002.00357.xinfo:eu-repo/semantics/openAccessArticle31313511841355Q2