Düzgün N.Esme H.Karabağli P.İyisoy M.S.Duran M.2020-03-262020-03-2620171300-0292https://dx.doi.org/10.5336/medsci.2017-55295https://hdl.handle.net/20.500.12395/35706Objective: Endogenous opioids play an important role in tolerance to ischaemia at cellular level. We aimed to investigate the effect of morphine sulphate on the histopathologic changes in the lung tissue after ischemia reperfusion injury. Material and Methods: Forty female Wistar Albino rats were used in the study. The subjects were divided into 4 groups. İschemia reperfusion (IR) group; thoracotomy was performed from the fifth left intercostal space and ischemia reperfusion injury was established. İschemia reperfusion and ischemic postconditioning (IRPC) group; thoracotomy, ischemia reperfusion and postcondutioning. IRPC3 and IRPC30 groups; in addition to IRPC group, 3 and 30 ?mol morphine sulphate were administered. In the histopathological examination of lung tissue, neutrophile count, congestion, interstitial edema, tissue damage, and VEGF (Vascular endothelial growth factor) and EGFR (Epidermal growth factor receptor) positively stained cells were evaluated semi quantitatively. Results: In the histopathological analysis there was a statistically significant difference between the IR with IRAK (P<0.01), IRAK3 (P<0.01), and IRAK30 (P<0.01) groups in terms of neutrophile count and congestion edema scores. There was a statistically significant difference between the IR with IRAK (P<0.01) and IRAK3 (P<0.01) groups and IRAK with IRAK30 (P<0.05) groups in terms of tissue damage score. Moreover there was a statistically significant difference between the IR and IRAK3 (P<0.01) and IRAK30 (P<0.01) groups in the histopathological analysis in terms of VEGF (+) and EGFR (+) staining cell scores. Conclusion: As a result, ischemic postconditioning and administration of morphine sulphate were applied in the ischemic reperfusion period and histopathologic evaluation was performed. It has been determined that administration of morphine sulphate in the prevention of ischemia reperfusion injury is a more effective method than ischemic postconditioning. © 2017 by Türkiye Klinikleri.tr10.5336/medsci.2017-55295info:eu-repo/semantics/openAccessMorphinePathologyReperfusion injuryArticle3727479Q4