Zengin, GökhanFerrante, ClaudioMenghini, LuigiOrlando, GiustinoBrunetti, LuigiRecinella, LuciaChiavaroli, AnnalisaLeone, SheilaRonci, MaurizioAumeeruddy, Muhammad ZakariyyahMahomoodally, Mohamad Fawzi2020-03-262020-03-262019Zengin, G., Ferrante, C., Menghini, L., Orlando, G., Brunetti, L., Recinella, L., Chiavaroli, A., Leone, S., Ronci, M., Aumeeruddy, M. Z., Mahomoodally, M. F. (2019). Protective Effects Of Cotoneaster İntegerrimus On İn Vitro And Ex‐Vivo Models Of H2O2‐İnduced Lactate Dehydrogenase Activity İn HCT116 Cell And On Lipopolysaccharide‐İnduced İnflammation İn Rat Colon. Journal Of Food Biochemistry, 43,(3), e12766.0145-88841745-4514https://dx.doi.org/10.1111/jfbc.12766https://hdl.handle.net/20.500.12395/38133The present study evaluated the biological potential of methanol and aqueous extracts of the twigs and fruits of Cotoneaster integerrimus Medik. Lethality bioassays performed on Artemia salina showed that aqueous and methanol C. integerrimus extracts were non-toxic in the concentration range (0.1-20 mg/ml), with a LC50 >= 2.5 mg/ml, for each single extract. The protective effect of the extracts was assessed in vitro against hydrogen peroxide-induced lactate dehydrogenase (LDH) activity and tumor necrosis factor (TNF)alpha gene expression in colon cancer HCT116 cell line. All the extracts downregulated (H2O2)-induced TNF alpha gene expression, in HCT116. By contrast, it was observed that the lipopolysaccharide (LPS)-induced increase in colon nitrite, prostaglandin E-2, and 8-iso-PGF(2 alpha) levels were counteracted mostly by the methanol twig extract. The present study showed protective effects induced by C. integerrimus in vitro and ex vivo, thus supporting potential application in the management of chronic inflammatory diseases.en10.1111/jfbc.12766info:eu-repo/semantics/openAccessantioxidantCotoneaster integerrimusinflammationnatural agentsoxidative stressArticle43331353569Q1WOS:000461068900021Q3