Maskovic, Pavle Z.Diamanto, Lazari D.Cvetanovic, AleksandraRadojkovic, MarijaSpasojevic, Miroslav B.Zengin, Gökhan2020-03-262020-03-2620161936-97511936-976Xhttps://dx.doi.org/10.1007/s12161-015-0321-5https://hdl.handle.net/20.500.12395/33862Different extraction conditions may significantly influence extraction of particular compound groups. In the present study, response surface methodology (RSM) based on Box-Behnken design was used to define the best combination of extraction temperature (20-60 A degrees C), ethanol concentration (10-90 %) and extraction time (60-180 min) for maximum yield of antioxidant compounds and maximum antioxidant activity of orange extracts. Experimental values of total phenol yields were in the range from 3.10 to 3.72 mgGAE/ml, while total flavonoid content was in the range from 1.42 to 2.13 mgRE/ml. Antioxidant activity expressed as the 50 % inhibition concentration (IC50 value) was in the range from 0.03 to 0.04 mg/ml. The experimental results were fitted to a second-order quadratic polynomial model, and they have shown a good fit to the proposed model (R (2) > 0.90). Determined optimized conditions for maximizing yield of antioxidant compounds were within the experimental range.en10.1007/s12161-015-0321-5info:eu-repo/semantics/closedAccessAntioxidantExtractionPhenolicsResponse surface methodologyArticle9514361443Q2WOS:000373636200039Q2