Tras, BunyaminCetin, GulUney, KamilDik, BurakCorum, OrhanAtalay, Sema2020-03-262020-03-262017Tras, B., Cetin, G., Uney, K., Dik, B., Corum, O., Atalay, S. (2017). Effects of BCRP and P-gp Modulators on the Penetration of Aflatoxin B1 into the Mouse Brain. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(1), 95-100.1300-6045https://dx.doi.org/10.9775/kvfd.2016.15904https://hdl.handle.net/20.500.12395/35031This study was conducted to determine whether the plasma and brain concentrations of AFB(1) are affected by the modulation of P-gp and BCRP using zosuquidar (ZQR) and prazosin (PRZ), respectively. In this study, a total of 40 healthy adult male BALB/c mice (32 +/- 3.7 g) were used. The animals were randomly divided into 5 groups, with 8 animals per group. Group 1 was used for method validation. Group 2 (AF) received intraperitoneal AFB1 at a dose of 20 mg/kg of body weight. Groups 3 (AF+PRZ), 4 (AF+ZQR), and 5 (AF+PRZ+ZQR) received 20 mg/kg of AFB(1) intraperitoneally 30 min after the intraperitoneal administration of prazosin (0.3 mg/kg), zosuquidar (25 mg/kg), and prazosin+zosuquidar (0.3 mg/kg prazosin + 25 mg/kg zosuquidar), respectively. Six hours after the administration of AFB(1), blood and brain samples were collected from the animals in Groups 2 to 5. AFB(1) concentrations were determined using an HPLC system with fluorescence detection. Individual and simultaneous administration of prazosin and zosuquidar significantly reduced the brain concentrations of AFB(1) in comparison to a single administration of AFB(1) (P<0.05). The brain/plasma ratio of the AF group was higher than that of the other groups (AF+PRZ, AF+ZQR, and AF+PRZ+ZQR) (P<0.05). Inducers of transmembrane proteins, especially BCRP, can be life saving during acute AFB(1) poisoning.en10.9775/kvfd.2016.15904info:eu-repo/semantics/openAccessAflatoxin B-1BrainDrug transporter proteinsModulationMiceArticle23195100Q3WOS:000388115200013Q4