Çelik, İ.Oğuz, H.Demet, O.Boydak, M.Dönmez, H. H.Sur, E.Nizamlıoğlu, F.2020-03-262020-03-262000Çelik, İ., Oğuz, H., Demet, O., Boydak, M., Dönmez, H. H., Sur, E., Nizamlıoğlu, F., (2000). Embryotoxicity Assay of Aflatoxin Produced by Aspergillus Parasiticus NRRL 2999. British Poultry Science, 41(4), 401-409. Doi: 10.1080/7136549610007-16681466-1799https://dx.doi.org/10.1080/713654961https://hdl.handle.net/20.500.12395/172131. The embryotoxicity of mixed aflatoxins (AF) and aflatoxin B-1 (AFB(1)) were evaluated by a modified chick embryotoxicity screening test (CHEST). Adverse effects on the early embryonic development of thymus and bursa of Fabricius were also investigated by light microscopy. AF consisted of 83.06% AFB(1), 12.98% AFB(2), 2.84% AFG(1) and 1.12% AFG(2). 2. A total of 448 fertilised laying hens' eggs were used. AF and AFB(1) were injected into the eggs at doses of 10, 100 and 1000 ng/egg. Embryonic developmental stages were evaluated according to the Hamburger-Hamilton scale (HH-scale). 3. The results showed that AFB(1) given at 10 ng/egg had a significantly (P<0.05) greater embryotoxic effect than AF given at a similar dose. The higher doses of both AF and AFB(1) caused higher embryonic mortality and also an increase in early deaths. 4. In the groups receiving 100 ng/egg AF and AFB(1) an abnormal development was seen, with a protruded central region, corresponding to the area pellucida of the blastoderm. No other developmental abnormality attributable to AF or AFB(1) was found.en10.1080/713654961info:eu-repo/semantics/openAccessArticle41440140911128380Q2WOS:000165512500003Q2