Güçlü, FeyzullahAldemir, Osman SelçukGüler, Leyla2020-03-262020-03-2620040035-1555https://hdl.handle.net/20.500.12395/18998Morphological identification of Sarcocystis spp. and the diagnosis of their developmental stages within the host is very difficult. However, recent developments in molecular biology have produced a number of techniques to overcome these difficulties. In this study, the RAPD-PCR (random amplified polymorphic DNA-PCR) technique was used to investigate the molecular biology of Sarcocystis spp. in cattle. In order to isolate the parasite, phenol extraction/ethanol precipitation method was employed to obtain parasitic DNA. After optimisation of the PCR conditions, five primers were selected to amplify the DNA of each Sarcocystis spp. As a result, various Sarcocystis spp. were detected and differentiated using this RAPD-PCR assay in cattle.eninfo:eu-repo/semantics/closedAccesssarcocystisdetectiondifferential identificationRAPD-PCRArticle15508.09.2020440444WOS:000224975000007Q4