Yaman, H.Ozturk, K.Uyar, Y.Gurbilek, M.2020-03-262020-03-2620080022-2151https://dx.doi.org/10.1017/S0022215107007724https://hdl.handle.net/20.500.12395/22351Objective: The aim of the present study was to investigate the dose-related effectiveness of corticosteroids in the management of otitis media with effusion, by using the objective assessment techniques of tympanometry and cytokine measurement. Materials and methods: The eustachian tubes of 42 male rats were obstructed. Thirty-six subjects with effusion were randomly divided into three equal groups: controls; 0.5 mg/kg/day corticosteroid; and 1 mg/kg/day corticosteroid. Concentrations of interleukin-1 beta and tumour necrosis factor a in the middle-ear effusions were analysed using enzyme-linked immunosorbent assay. Results: An effusion occurred on the 14th post-operative day and was present on the 30th post-operative day in the control group. Otomicroscopic examination and tympanometric measurement showed an improvement in the intervention groups. There was a significant difference between both intervention groups and the control group for both interleukin-1 beta and tumour necrosis factor alpha concentrations (p < 0.01). However, there was no significant difference between the 0.5 mg/kg and the I mg/kg corticosteroid groups (p > 0.05). Conclusion: The study confirmed a short term beneficial effect of systemic steroids on resolution of otitis media with effusion in a rat model. Our results support the theory that corticosteroids significantly decrease concentrations of both interleukin-1 beta and tumour necrosis factor alpha. In our model, the 0.5 mg/kg corticosteroid dose was as effective as the 1 mg/kg corticosteroid dose. Further studies should be carried out to determine the optimal dose and duration of corticosteroids in the treatment of otitis media with effusion.en10.1017/S0022215107007724info:eu-repo/semantics/closedAccessotitis media with effusionacoustic impedence teststumour necrosis factor-alphainterleukinescorticosteroidsArticle1221253017445334Q2WOS:000252864600006Q4