Sariozkan, SerpilBucak, Mustafa NumanTuncer, Purhan BarbarosBuyukleblebici, SerhatEken, AyseAkay, Cemal2020-03-262020-03-2620150011-22401090-2392https://dx.doi.org/10.1016/j.cryobiol.2015.04.011https://hdl.handle.net/20.500.12395/32236It was determined that fetuin and hyaluronan supplementation did not provide any significant effect on the post-thaw subjective and CASA motility percentages and sperm motion characteristics, in comparison to the controls (P > 0.05). Sperm acrosome and total abnormalities were similar in all groups (P > 0.05). Groups M (hyaluronan + fetuin) and H (hyaluronan) displayed a higher rate of sperm membrane integrity, compared to that of Group C (control) (P < 0.01). According to the results of the comet assay, the lowest percentage of sperm with damaged DNA was achieved in Group H, when compared to all of the experimental groups (P < 0.01). Furthermore, all of the additives resulted in a lower rate of sperm with damaged DNA than that of the controls, and thus, reduced DNA damage (P < 0.01). For pregnancy rates, there were no significant differences between the extender groups (P > 0.05). MDA formation was found to be lower in Groups M and F (P < 0.01). In Group M, SOD activity was determined to have significantly increased (23.61 +/- 5.62 U/ml) compared to the other groups (P < 0.01). All experimental groups had a GSH-Px activity higher than that of the control group (P < 0.01). Crown Copyright (C) 2015 Published by Elsevier Inc. All rights reserved.en10.1016/j.cryobiol.2015.04.011info:eu-repo/semantics/closedAccessBullAntioxidantsSperm parametersBiochemical parametersDNA damageFreeze-thawingArticle71111912425962321Q1WOS:000358632700017Q2