Uysal, AhmetZengin, GökhanMollica, AdrianoGüneş, ErdoğanLocatelli, MarcelloYılmaz, TurgutAktümsek, Abdurrahman2020-03-262020-03-2620160944-7113https://dx.doi.org/10.1016/j.phymed.2016.06.011https://hdl.handle.net/20.500.12395/33347Background: The Cotoneaster species are widely used as traditional purposes in different countries including Turkey. Purpose: The study was performed to evaluate the biological and chemical profile of two extracts (methanol (T-Me; F-Me) and water (T-W; F-W)) from two parts (twigs and fruits) of Cotoneaster integerrimus. Materials and methods: Antioxidant (free radical scavenging (DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating), enzyme inhibitory (cholinesterase, tyrosinase, alpha-amylase and alpha-glucosidase), antimicrobial (standard microorganisms and methicillin-resistant Staphylococcus aureus isolates (MRSA)) and mutagenic/antimutagenic effects (by Ames assay) were tested for biological profile. For chemical profile, total and individual phenolic components were detected for each extract. Results: Generally, T-Me reflected the strongest biological effects with the highest level of phenolics (115. 15 mgGAEs/g extract). Also, twig extracts had more potent biological effects as compared to flower extracts. Eight-teen phenolics were identified in the extracts. (-)- epicatechin was the major constituent in all extracts and is mainly responsible for biological activities observed. Its amount present in F-W and T-W were 9.27 and 32.89 mg/g extract, respectively. Also, molecular docking was used to understand enzyme-epicatechin interactions. Conclusion: From these results, this plant has a great potential as a health promoter for developing novel functional food ingredients and pharmaceutical preparations. (C) 2016 Elsevier GmbH. All rights reserved.en10.1016/j.phymed.2016.06.011info:eu-repo/semantics/closedAccessCotoneaster(-)-epicatechinBiological effectChemical fingerprintMolecular dockingNutraceuticalsArticle231097998827444342Q1WOS:000379834500001Q1