Baş, A. L.Elmas, M.Şimşek, A.Traş, B.Yazar, E.Hadimli, H. H.2020-03-262020-03-262000Hadimli, H. H., Yazar, E., Traş, B., Şimşek, A., Elmas, M., Baş, A. L., (2000). Efficacies of Free and Liposome-Encapsulated Enrofloxacin (Baytril (R)) Against Staphylococcus Aureus Infection in Turkish Shepherd Dog Neutrophils in Vitro. Revue De Medecine Veterinaire, 151(5), 415-420.0035-1555https://hdl.handle.net/20.500.12395/17211The influence of free and liposome-encapsulated enrofloxacin at concentrations of 1, 2 and 4 mu g/ml was studied on phagocytic and intracellular killing of Staphylococcus aureus, in cultured neutrophils of six healthy Turkish Shepherd Dogs. Enrofloxacin was encapsulated by using reverse-phase evaporation method in multilamellar liposomes composed of 15 mg egg phosphatidylcholine and 35 mg cholesterol. Phagocytic and killing activity of neutrophils were determined by fluorescent microscopic technique. Liposome-encapsulated enrofloxacin significantly enhanced the intraphagocytic killing of bacteria in neutrophils compared to free drug. Phagocytic activity of neutrophils was not significantly different among groups. The results obtained in the present study show that use of enrofloxacin encapsulated in liposomes may improve the antibacterial efficacy of the antibiotic.L’activité bactéricide des neutrophiles de chiens de berger turcs, traités par l'enrofloxacine et par l'enrofloxacine encapsulée vis-à-vis de Staphylococcus aureus fait l’objet de cette étude. La phagocytose et l’activité bactéricide des neutrophiles ont été évaluées par fluorescence. L'enrofloxacine encapsulée augmente le pouvoir bactéricide de l’enrofloxacine, alors que l’activité phagocytaire n’est pas modifiée. Ces résultats montrent une meilleure efficacité de l’enrofloxacine encapsulée.eninfo:eu-repo/semantics/openAccessenrofloxacinintracellular bioactivityneutrophileStaphylococcus aureusTurkish Shepherd DogliposomeneutrophileArticle1515415420WOS:000087999200004Q2