Oztekin, YaseminRamanaviciene, AlmiraYazicigil, ZaferSolak, Ali OsmanRamanavicius, Arunas2020-03-262020-03-2620110956-56631873-4235https://dx.doi.org/10.1016/j.bios.2010.11.001https://hdl.handle.net/20.500.12395/26335This study reports direct electron transfer (DET) from immobilized glucose oxidase (GOx) via grafted and electropolymerized 1,10-phenanthroline monohydrate (PMH). The layer of poly-1,10-phenanthroline (PPMH) was gained via electrochemical deposition, which was used to create the PPMH-modified GC-electrode (PPMH/GC-electrode). Further, the GOx was immobilized on the PPMH/GC-electrode. The effect of surface-modification by the PPMH on the electron-transfer between enzyme and electrode-surface and some other electrochemical/analytical-parameters of newly designed enzymatic-electrode were evaluated. The PPMH/GC-electrode showed superior DET to/from flavine adenine dinucleotide cofactor of GOx, while some redox-compounds including ferrocene and K-3[Fe(CN)(6)] were completely electrochemically inactive on the PPMH/GC-electrode. It was also found that the resulting GOx/PPMH/GC-electrode functioned as a "direct response type" glucose-biosensor. The biosensor showed excellent selectivity towards glucose and demonstrated good operational-stability. According to our best knowledge, this study is the first scientific report on electrochemical-polymerization of PMH on the GC-electrode in non-aqueous media followed by its application in the design of glucose-biosensor. (C) 2010 Elsevier B.V. All rights reserved.en10.1016/j.bios.2010.11.001info:eu-repo/semantics/closedAccessSurface-modificationPhenanthrolineGlucose oxidaseBiosensorElectron transferElectrocatalysisArticle2652541254621146394Q1WOS:000286904400121Q1