Hakkı, S. SemaBozkurt, S. BuketHakkı, Erdoğan E.Korkusuz, PetekPuralı, NuhanKoç, NursenTimuçin, MuharremÖztürk, AdnanKorkusuz, Feza2020-03-262020-03-262012S. Hakkı, S., Bozkurt, S. B., Hakkı, E. E., Korkusuz, P., Puralı, N., Koç, N., Timuçin, M., Öztürk, A., Korkusuz, F., (2012). Osteogenic Differentiation of MC3T3-E1 Cells on Different Titanium Surfaces. Biomedical Materials, 7(4), 1-9. Doi:10.1088/1748-6041/7/4/0450061748-60411748-605Xhttps://dx.doi.org/10.1088/1748-6041/7/4/045006https://hdl.handle.net/20.500.12395/28281mRNA expressions related to osteogenic differentiation of MC3T3-E1 cells on electro-polished smooth (S), sandblasted small-grit (SSG) and sandblasted large-grit (SLG) surfaces of titanium alloys were investigated in vitro. Gene expression profiles of cells were evaluated using the RT2 Profiler PCR microarray on day 7. Mineralizing tissue-associated proteins, differentiation factors and extracellular matrix enzymes mRNA expressions were measured using Q-PCR. SLG surface upregulated 23 genes over twofolds and downregulated 3 genes when compared to the S surface. In comparison to the SSG surface, at least a twofold increase in 25 genes was observed in the SLG surface. BSP, OCN, OPN, COL I and ALP mRNA expressions increased in the SLG group when compared to the S and the SSG groups. BMP-2, BMP-6 and TGF-beta mRNA expressions increased in both the SSG and the SLG surfaces. MMP-2 and MMP-9 mRNA expressions increased as the surface roughness increased. This study demonstrated that surface roughness of titanium implants has a significant effect on cellular behavior and SLG surface apparently increased gene expressions related to osteogenesis when compared to the S and the SSG surfaces.en10.1088/1748-6041/7/4/045006info:eu-repo/semantics/openAccessArticle741922570189Q2WOS:000306712900006Q2