Ustun, MEBariskaner, HYosunkaya, AGurbilek, MDogan, N2020-03-262020-03-2620040953-14241952-4021https://hdl.handle.net/20.500.12395/19037In the present study, the effects of magnesium sulfate on Na+,K+-ATPase levels and intracranial pressure (ICP) after cerebral ischemia in rabbits were studied. Thirty New Zealand rabbits were divided into three groups. Group I was the control group. In group 2 (untreated group) cerebral ischemia was produced by clamping bilateral common carotid arteries for 60 min but in group 3 magnesium sulfate was administered 100 mg/kg i.v. 10 min after opening the clamps. In group 1, ICP recordings were obtained 5, 60 and 120 min after craniectomy. In groups 2 and 3, ICP recordings were obtained 5 min after craniectomy but before clamping, 60 min after clamping and 60 min after opening the clamps. After taking ICP recordings, brain cortices were resected and Na+,K+-ATPase activity was determined by subtracting the enzyme activity in the presence of ouabain from the total activity in the absence of ouabain method. There was a significant difference between Na+,K+-ATPase levels of ;group 1 and group 2 (P < 0.05). There was no significant difference in Na+,K+-ATPase levels between group 1 and 3 (P > 0.05), also preischemic ICP values were same in all groups (P > 0.05). Preischemic and postischemic ICP values were significantly different between groups 1 and 2 (P < 0.05), also postischemic (120 min) ICP values were significantly different between group 2 and group 3 (P < 0.05). ICP values correlate well with Na+,K+-ATPase level. These results demonstrate that cerebral ischemia leads to a decrease of ATPase level in the brain and magnesium sulfate suppresses the decrease of Na+,K+-ATPase, also magnesium sulfate treatment improves the ICP changes.eninfo:eu-repo/semantics/closedAccessmagnesium sulfatecerebral ischemiaNa+-K+-ATPaserabbitArticle17316917515724864WOS:000225370900003Q4