Corum, Orhan.Terzi, Ertugrul.Corum, Duygu Durna.Kenanoglu, Osman Nezih.Bilen, Soner.Uney, Kamil.2020-03-262020-03-262020Corum, O., Terzi, E., Corum, D. D., Kenanoglu, O. N., Bilen, S., Uney, K. (2020). Pharmacokinetic/Pharmacodynamic Integration of Marbofloxacin After Oral and Intravenous Administration in Rainbow Trout (Oncorhynchus mykiss). Aquaculture, 514, 1-5.0044-84861873-5622https://dx.doi.org/10.1016/j.aquaculture.2019.734510https://hdl.handle.net/20.500.12395/38579The pharmaco-kinetic/dynamic of marbofloxacin was investigated after single intravenous (IV) and oral administration of 10 mg/kg in 192 healthy rainbow trout at 13 +/- 1.2 degrees C. The plasma concentrations of marbofloxacin were determined by high-performance liquid chromatography-ultraviolet detection. After IV and oral administration, the plasma concentration-time data were described by a noncompartmental analysis. The minimal inhibitory concentration (MIC) of marbofloxacin against Yersinia ruckeri, Aeromonas hydrophila, Pseudomonas fluorescens and P. putida were determined by broth dilution method at 13 degrees C. After IV administration, the elimination half-life (t(1/2)(lambda z)), area under the concentration-versus time curve (AUC(0-infinity)), apparent volume of distribution at steady-state and total body clearance of marbofloxacin were 18.05 h, 354.63 h * mu g/mL, 0.65 L/kg and 0.03 L/h/kg, respectively. After oral administration, t(1/2 lambda z), AUC(0)(-infinity) the peak plasma concentration, time of maximum concentration and bioavailability were 27.51 h, 135.29 h * mu g/mL, 3.74 mu g/mL, 4 h and 38.15%, respectively. The respective MICs of marbofloxacin against Y. ruckeri, A. hydrophila, P. fluorescens and P. putida were determined as 0.02 mu g/mL, 2.5 mu g/mL, 2.5 mu g/mL and 5 mu g/mL, respectively. Following IV and oral administration of 10 mg/kg marbofloxacin, AUC/MIC and C-max/MIC values were above the target levels for Y. ruckeri, while this dose was not sufficient for A. hydrophila and Pseudomonas spp. Because the pharmacokinetics and pharmacodynamics of a drug in fish are significantly affected by temperature, the dosage regimen of marbofloxacin should be modified according to temperature.en10.1016/j.aquaculture.2019.734510info:eu-repo/semantics/openAccessPharmacokineticsPharmacodynamicsMarbofloxacinRainbow troutArticle514Q1WOS:000495358600031Q1