Shrestha, Shyam SharanSut, StefaniaDi Marco, Serena BarbonZengin, GökhanGandin, ValentinaDe Franco, MichelePant, Deepak RajMahomoodally, Mohamad FawziDall'Acqua, StefanoRajbhandary, Sangeeta2020-03-262020-03-262019Shrestha, S. S., Sut, S., Barbon Di Marco, S., Zengin, G., Gandin, V., De Franco, M., Pant, D.R., Mahomoodally, M. F., Dall'Acqua, S., Rajbhandary, S. (2019). Phytochemical Fingerprinting And İn Vitro Bioassays Of The Ethnomedicinal Fern Tectaria Coadunata (J. Smith) C. Christensen From Central Nepal. Molecules, 24,(24), 4457.1420-3049https://dx.doi.org/10.3390/molecules24244457https://hdl.handle.net/20.500.12395/38109Tectaria coadunata, an ethnomedicinal fern used in Nepal to treat a large number of diseases, has been poorly studied with regard to its phytochemical composition and possible bioactivity. This study was performed with the aim of supporting traditional medicine as a new source of bioactive constituents. Phytochemical compositions of methanol extracts were determined by nuclear magnetic resonance (NMR), liquid chromatography-diode array detector-mass spectrophotometry (LC-DAD-MS), and liquid chromatography-fluorescence-mass spectrometry. Quali-quantitative data revealed large amount of procyanidins, mainly of the A-type, as well as eriodictyol-7-O-glucuronide and luteolin-7-O-glucoronide as main constituents. The antioxidant, cytotoxic, and inhibitory activity of five enzymes that are implicated in human diseases was evaluated for the extract and fractions. High free-radical scavenging activity in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays and inhibitory activities against cholinesterases and tyrosinase were observed. Furthermore, a moderate cytotoxic effect was observed on the 2008 and BxPC3 cell lines. Overall results showed potential usefulness of this fern as a source of phytochemicals for pharmaceutical uses.en10.3390/molecules24244457info:eu-repo/semantics/openAccessTectaria coadunataantioxidantmedicinal fernchromatographyprocyanidinsflavonolsArticle242431817382Q1WOS:000507299600035Q2