Sahin, Ayse SaideBariskaner, Hulagu2020-03-262020-03-2620070767-39811472-8206https://dx.doi.org/10.1111/j.1472-8206.2007.00541.xhttps://hdl.handle.net/20.500.12395/21694In the present study, we analysed the effects of leptin on rabbit aorta and the mechanisms underlying these effects. Leptin (10(-12)-10(-9) M) induced concentration-dependent relaxation in intact rabbit aorta rings precontracted with phenylephrine (10(-6) M). Removal of endothelium abolished the effects of leptin. Pretreatment of rings with N-omega-nitro-L-arginine methyl ester (10(-4) M 20 min) or catalase (1200 U/mL 20 min) significantly reduced the relaxant response to leptin when compared with the control group. The incubation of brefeldin A (3.5 x 10(-5) M 90 min), indomethacin (10(-5) M 20 min), tetraethylammonium (10(-4) M 20 min), and glibenclamide (10(-5) M, 20 min) did not affect the leptin-induced vasodilation. These results suggest that leptin relaxes the rabbit aorta. The relaxation is mediated by endothelium-derived nitric oxide and hydrogen peroxide.en10.1111/j.1472-8206.2007.00541.xinfo:eu-repo/semantics/closedAccessH2O2leptinnitric oxiderabbit aortaArticle21659560018034660Q2WOS:000251189500004Q2