Yurtçu, MüslimAbasıyanık, AdnanAvunduk, Mustafa CihatKaragözoğlu, EmrahAbasıyanık, Fatma2020-03-262020-03-2620051300-0292https://hdl.handle.net/20.500.12395/19992Objective: The aim of the study is to determine the effects of melatonin and steroid treatments which were injected as one-dose and one-dose for seven days to prevent ischemia-reperfusion injury occurred in testicle after torsion and detorsion and which of these treatments is more effective. Material and Methods: Eighty prepubertal male Sprague-Dawley rats were devided into 8 groups each containing 10 rats. In all groups, except sham groups, torsion was created by rotating the left testicles 720° in a clockwise direction for 6 hours. In melatonin groups 17 mg/kg melatonin and in steroid groups 1 mg/kg methylprednesolone were injected before 30 minutes from detorsion. In seven dose steroid and melatonin groups melatonin and methylprednesolone were injected once a day for 7 days. In Sham-1, torsion-detorsion-1, one-dose melatonin and, one-dose steroid groups left orchiectomies were created after 6 hours of detorsion. In other group, left orchiectomies were performed 7 days later. After performing histopathologic examination we determined MDA levels and Johnsen's scores of testicular tissue to identify ischemia-reperfusion injury. Results: MDA levels in one-dose melatonin and steroid groups were significantly less compared with torsion-detorsion-1 group (p < 0.05). Johnsen's scores were the same as in one-dose melatonin, steroid and torsion-detorsion-1 groups (p > 0.05). It was identified that MDA levels in seven dose melatonin and seven dose steroid groups were significantly less compared with torsion-detorsion-2 group (p < 0.01) and Johnsen's scores were significantly higher compared with torsion-detorsion-2 group (p < 0.01). Conclusion: The treatments of one-dose melatonin and steroid are effective against the I-R damage biochemically, but not histopathologically. Whereas, melatonin and steroid, which were injected once a day for 7 days, prevent ischemia-reperfusion injury completely. Copyright © 2005 by Türkiye Klinikleri.trinfo:eu-repo/semantics/closedAccessMelatoninReperfusion injurySpermatic cord torsionSteroidsArticle254496500Q4