Bas, Salih Zeki2020-03-262020-03-2620141759-96601759-9679https://dx.doi.org/10.1039/c4ay01524jhttps://hdl.handle.net/20.500.12395/30540A new biosensor based on immobilization of glucose oxidase on a multiwalled carbon nanotube-gold nanoparticle coated glassy carbon electrode (GCE) was fabricated for the electrochemical determination of glucose. First, a carboxylated multiwalled carbon nanotube (cMWCNT) was functionalized with 4-aminothiophenol (ATP) by a coupling agent, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, and then gold nanoparticles (AuNPs) were covalently linked to the ATP-cMWCNT via the formation of a covalent Au-S bond. Finally, glucose oxidase was immobilized simultaneously by the electropolymerization of o-phenylenediamine on the AuNP-ATP-cMWCNT coated GCE. The biosensor showed a linear range from 0.05 mM to 8.85 mM with a sensitivity of 27.93 mu A mM(-1) cm(-2) and a detection limit of 0.015 mM for the detection of glucose. The biosensor was successfully applied to estimate the glucose concentration in human blood serum samples. Furthermore, the AuNP-ATP-cMWCNT was investigated for the non-enzymatic detection of hydrogen peroxide by using voltammetric and amperometric techniques, indicating a linear relationship in the range of 5 x 10(-3) to 13.10 mM.en10.1039/c4ay01524jinfo:eu-repo/semantics/closedAccessArticle61977527759Q2WOS:000342178500024Q2