Ulker, M.Ulker, H. E.Botsali, M. S.Dundar, A.2020-03-262020-03-2620181119-3077https://dx.doi.org/10.4103/njcp.njcp_134_18https://hdl.handle.net/20.500.12395/36575Objectives: The aim of this study was to evaluate the efficacy of using erbium, chromium-doped: yttrium scandium gallium garnet (Er,Cr:YSGG) laser-treated dentine in a dentine barrier test device. Materials and Methods: The test materials (G-Bond (TM) and Vitrebond (TM)) were applied onto laser-treated or laser-untreated dentine discs. After 24 h of exposure with perfusion of the test chamber, cell survival was evaluated based on enzyme activity and compared to a nontoxic control material. The mean of the control was set to 100% viability. Data were analyzed using the one-way analysis of variance and the Tukey's honest significant difference tests. Results: The responses of bovine pulp-derived cells after exposure to G-Bond and Vitrebond on Er, Cr: YSGG laser-treated and laser-untreated dentin were statistically different from negative control group (P < 0.05). Conclusion: Er,Cr:YSGG laser treatment was not successful enough in decreasing the cytotoxic effects of the dental materials. Different parameters of Er,Cr:YSGG laser or different laser types could be investigated as an alternative to minimizing the cytotoxic effects of dental materials.en10.4103/njcp.njcp_134_18info:eu-repo/semantics/closedAccessCytotoxicitydentine barriererbiumchromium-doped:yttrium scandium gallium garnet laserArticle21101311131630297564Q3WOS:000446795500012Q4