Acar, HasanConnor, Micheal J.2020-03-262020-03-261998Acar, H., Connor, M. J., (1998). Detection of Trisomy 12 and Centromeric Alterations in CLL by Interphase- and Metaphase-Fish. Cancer Genetics and Cytogenetics, 100(2), 148-151. doi: 10.1016/S0165-4608(97)00024-10165-4608https://dx.doi.org/10.1016/S0165-4608(97)00024-1https://hdl.handle.net/20.500.12395/16755We have studied trisomy 12 in chronic lymphocytic leukemia (CLL) by fluorescence in situ hybridization (FISH) with an alpha-satellite centromeric probe for chromosome 12 on both dividing and non-dividing cells. Trisomy for chromosome 12 tvas demonstrated in four of these patients (15.3%) using FISH on interphase cells. The percentage of trisomic cells ranged from 10% to 65% of nuclei. The hybridization signals in the trisomic and disomic nuclei were of a broadly similar size and nature. Interestingly, three of the remaining CLL patients, who exhibited disomy for chromosome 12, showed a marked difference in size of the hybridization signals in interphase nuclei. This was also demonstrated in metaphase spreads. In addition, metaphase FISH studies revealed a supernumerary marker chromosome in three out of 26 patients with CLL.en10.1016/S0165-4608(97)00024-1info:eu-repo/semantics/openAccessDetection of Trisomy 12 and Centromeric Alterations in CLL by Interphase- and Metaphase-FishArticle10021481519428359N/AWOS:000071133300009Q2