Maltas, EsraMalkondu, SaitUyar, PembegulOzmen, Mustafa2020-03-262020-03-2620150928-49311873-0191https://dx.doi.org/10.1016/j.msec.2014.11.057https://hdl.handle.net/20.500.12395/32172N,N'-Bis[tris-(2-aminoethyl) amine]-3,4,9,10-perylenetetracarboxylic diimide (PBI-TRIS), nonfluorescent dye was used to fluorescent labelling of DNA. For this aim, (3-aminopropyl) triethoxysilane (APTS) modified superparamagnetic iron oxide nanopartides (SPIONs) were synthesized to provide a suitable surface for binding of DNA. Amine functionalized nanoparticles showed a high immobilization capacity (82.70%) at 25 mg of nanoparticle concentration for Calf thymus DNA. Binding capacity of PBI-TRIS to DNA-SPION was also found as 1.93 mu M on 25 mg of nanoparticles by using UV-vis spectroscopy. Binding of PBI-TRIS to DNA onto nanoparticles was also characterized by scanning electron microscopy and infrared spectroscopy. The confocal images of PBI-TRIS labelled DNA-SPION and breast cells were taken at 488 and 561.7 nm of excitation wavelengths. Cell image was also compared with a commercial dye, DAPI at 403.7 nm of excitation wavelength. Results showed that PBI-TRIS can be used for cell staining. (C) 2014 Elsevier B.V. All rights reserved.en10.1016/j.msec.2014.11.057info:eu-repo/semantics/closedAccessCalf thymus DNACell imageFluorescent labellingPerylene bisimideArticle48869325579900Q1WOS:000348749200012Q2