Maltaş, EsraÖzmen, MustafaYıldız, SalihErsöz, Mustafa2020-03-262020-03-262012Maltas, Esra., Özmen, Mustafa., Yıldız, Salih., Ersöz, Mustafa., (2012). Binding Affinity of Serum Proteins to Epoxy Modified Magnetite Nanoparticles. Advanced Science Letters, 17(1), 143-148.1936-6612https://dx.doi.org/10.1166/asl.2012.4242https://hdl.handle.net/20.500.12395/28736Superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution. SPIONs modified with [3-(2,3-epoxypropoxy)propyl] trimethoxy silane, which resulted in the presence of a high population of epoxy groups on the particles surface. The surface functional SPIONs can be bound with active bio-substance, and have a wide application prospect in the fields of biology and medicine. The binding percentage of proteins was studied with epoxy modified SPIONs. Several serum proteins including albumin, ?-globulin, hemoglobin, cytochrome c and myoglobin were studied for immobilization. Epoxy terminated SPIONs were shown the highest binding ability for immobilization of cytochrome c and hemoglobin with 1 and 0.86 mg total protein at a particle concentration of 20 mg/mL among proteins, respectively. This binding ability was also shown by using sodium dodecylsulfate polyacrylamide gel electrophoresis technique (SDS-PAGE). Protein attachments were also examined by Scanning Electron Microscopy (SEM). © 2012 American Scientific Publishers. All rights reserved.en10.1166/asl.2012.4242info:eu-repo/semantics/closedAccessCytochrome CEpoxyHemoglobineMagnetic NanoparticlesSds-PageBinding Affinity of Serum Proteins to Epoxy Modified Magnetite NanoparticlesArticle171143148N/A