Özyılmaz, ElifSayın, SerkanArslan, MustafaYılmaz, Mustafa2020-03-262020-03-2620140927-77651873-4367https://dx.doi.org/10.1016/j.colsurfb.2013.08.019https://hdl.handle.net/20.500.12395/30919A silica-based p-cyclodextrin was immobilized on magnetic nanoparticles to obtain a macrocyclic compound with magnetic property. Then, the beta-cyclodextrin-grafted magnetic nanoparticles were encapsulated with Candida rugosa lipase in sol-gel matrices using alkoxysilane precursors. The catalytic activity of the encapsulated lipases was evaluated with model reactions, i.e., the hydrolysis of p-nitro-phenylpalmitate (p-NPP) and the enantioselective hydrolysis of rasemic Naproxen methyl ester that was studied in an aqueous buffer solution/isooctane reaction system. The results indicate that the cyclodextrin-based, encapsulated lipase particularly exhibited high conversion and enantioselectivity behavior compared to the sol-gel free lipase. It was also observed that excellent enantioselectivity (E=399) was obtained for the encapsulated lipase with magnetic beta-cyclodextrin that has an ee value of S-Naproxen acid of about 98%. (C) 2013 Elsevier B.V. All rights reserved.en10.1016/j.colsurfb.2013.08.019info:eu-repo/semantics/closedAccessLipaseCyclodextrinFe3O4EnantioselectivityDrugArticle11318218924090713Q1WOS:000331670200023Q1