Atacan, KezibanKursunlu, Ahmed NuriOzmen, Mustafa2020-03-262020-03-262019Atacan, K., Kursunlu, A. N., Ozmen, M. (2019). Preparation Of Pillar [5] Arene İmmobilized Trypsin And İts Application İn Microwave-Assisted Digestion Of Cytochrome C. Materials Science And Engineering: C, 94, 886-893.0928-49311873-0191https://dx.doi.org/10.1016/j.msec.2018.10.043https://hdl.handle.net/20.500.12395/38120This work presents the immobilization of trypsin on the synthesized pillar[5]arene derivative (P5) containing ten iodo-terminals. The characterization has been carried out by Thermogravimetric Analysis (TGA), Fourier Transform Infrared (FTIR) and Raman spectra, Scanning Electron Microscopy (SEM) and Zeta Potential. Furthermore, Cytochrome c (Cyt c) was chosen as a model protein for evaluation of the performance of the pillar [5]arene-immobilized trypsin (P5-T), and its microwave-assisted digestion conditions were investigated by using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for 15 second. The peptide numbers of 10 and sequence coverage of 93% for 15 second microwave-assisted digestion were obtained for P5-T. The results can be further applied in future proteomics studies due to high efficiency, good reproducibility and stability of the pillar[5]arene-immobilized trypsin.en10.1016/j.msec.2018.10.043info:eu-repo/semantics/openAccessPillar[5]areneTrypsinImmobilizationCytochrome cMicrowave digestionArticle9488689330423776Q1WOS:000451360300088N/A