Kizmaz, MuhammetMarakoglu, KamileKiyici, AyselAy, Emre2020-03-262020-03-2620160009-91201873-2933https://dx.doi.org/10.1016/j.clinbiochem.2016.02.011https://hdl.handle.net/20.500.12395/33883Objectives: Netrin-1 is a laminin like protein that has recently been suggested to involve in the pathophysiology of atherosclerosis and treatment targets. The relationship between netrin-1 and atherosclerosis has not been evaluated in a human research previously and the effect of smoking on plasma netrin-1 levels as well. In this study, we aimed to evaluate plasma netrin-1 levels in smokers and non-smokers and the impact of smoking on netrin-1 levels. Our second aim was to evaluate the existence of peripheral artery disease in these groups by means of ankle brachial index (ABI) measurements and the relation between plasma netrin-1 levels and peripheral artery disease. Design and Methods: Smoker and non-smoker male patients were contributed in the study, 112 males in each group. ABI of all patients was determined with an automatic device and plasma netrin-1 levels were quantified with an enzyme-linked immunosorbent assay. Netrin-1 levels were compared between smoker and non-smoker cases, and the cases with and without peripheral arterial disease. Results: Plasma netrin-1 levels of smokers (4.54 +/- 2.87 pg/mL) were significantly higher than in non-smokers (3.81 +/- 1.26 pg/mL) (p = 0.015). Similarly, netrin-1 levels of cases with peripheral arterial disease (6.21 +/- 2.16 pg/mL) were significantly higher than the ones without peripheral arterial disease (4.07 +/- 2.16 pg/mL) (p = 0.002). Amount of smoking (packet.year) and ABI were found to be significantly related with netrin-1 levels. The relationship between PAD and netrin-1 levels remained significant after adjusting for smoking status. Conclusion: We can conclude that plasma netrin-1 levels significantly increase in smokers and peripheral artery disease is significantly related with netrin-1 levels after adjusting for smoking status. (C) 2016 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.en10.1016/j.clinbiochem.2016.02.011info:eu-repo/semantics/closedAccessNetrin-1Ankle brachial indexSmokingAtherosclerosisArticle4910.11.202083283426944719Q2WOS:000378221800018Q2