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Öğe Epidermal Growth Factor (Egf) and Heparin Binding-Egf (Hb-Egf) Mrna Expressions in Cyclic and Pregnant Mare Endometrium(WILEY-BLACKWELL PUBLISHING, INC, 2010) Atlı, Mehmet Osman; Kurar, Ercan; Kayış, Seyit Ali; Aslan, Selim; Semacan, Ahmet; Çelik, Sefa; Güzeloğlu, Aydın[Abstract not Available]Öğe Evaluation of Genes Involved in Prostaglandin Action in Equine Endometrium During Estrous Cycle and Early Pregnancy(Elsevier Science Bv, 2010) Atlı, Mehmet O.; Kurar, Ercan; Kayış, Seyit Ali; Aslan, Selim; Semacan, Ahmet; Çelik, Sefa; Güzeloğlu, AydınThe aim was to evaluate expression of genes involved in the biosynthesis of prostaglandins (PTG), Prostaglandin H Synthase-1 (PTGS1) and PTGS2, PGF synthase (PTGFS), and PGE synthase (PTGES), PGF receptor (PTGFR). PGE receptors (PTGER2 and FTGER4), prostaglandin transporter (SLCO2A1) and hydroxyprostaglandin dehydrogenase-15 (HPGD). Endometrial biopsies were obtained from mares on day of ovulation (d0, n = 4), late diestrus (LD, n = 4), early luteolysis (EL n = 4) and after luteolysis (AL n = 4) during the cycle. Stages of the cycle were confirmed by plasma progesterone concentrations measured daily and ultrasound examinations. Biopsies were also taken on days 14 (P14; n = 4), 15 (P15. n = 4), 18 (P18, n = 4) and 22 (P22; n = 4) of pregnancy. Relative mRNA expressions were quantified using real-time RT-PCR. A mixed model was fitted on the normalized data and least significant difference test (alpha = 0.05) was employed. Expression of PTGS1 mRNA was low throughout the estrous cycle and early days of pregnancy, but upregulated on P18 and P22. PTGS2 expression was increased on EL but it was suppressed by pregnancy on P15, P18, and P22. PTGFS expression was upregulated in both cyclic and pregnant mares compared to d0 and its level was the highest on LD. PTGFR expression was transiently increased on LD and EL and was suppressed during early pregnancy. Both PTGES and PTGER2 expressions were increased on LD, El, and early pregnancy, but were decreased after the luteolysis in cyclic mares as they remained high on P18 and P22. PTGER4 expression did not change throughout the cycle and early pregnancy. Levels of HPGD and SLCO2A1 were significantly increased only on P22. In conclusion, PTGS2 expression increases around the time of luteolysis and concurrent upregulation of PTGFS and PTGES indicates that equine endometrium has increased capability of PTG production around the time of luteolysis. However, pregnancy reduces PTGS2 expression, but maintains the high levels of PTGES during early pregnancy along with PTGER2 while PTGFR expression was suppressed. These findings suggest that possible luteotrophic action of PGE(2) is required in early equine pregnancy. PTGS1 is only upregulated later in the early pregnancy suggesting that it is not involved in luteolysis, but could be the main PTGS enzyme at this time during early pregnancy. An increase in HPGD and SLCO2A1 levels on P22 indicates a tight regulation of PTG action by pregnancy.Öğe Expression of Toll-like Receptors (Tlrs) is Regulated by Early Pregnancy in the Mare Endometrium.(SOC STUDY REPRODUCTION, 2012) Atlı, Mehmet Osman; Kurar, Ercan; Kayış, Seyit Ali; Aslan, Selim; Semacan, Ahmet; Çelik, Sefa; Güzeloğlu, AydınPractical, reliable and economical pregnancy diagnosis method for as early as possible time of pregnancy in mares is still requirement. Immune tolerance against allogenic fetus is critically important during the early pregnancy. Tolllike Receptors (TLRs) are known to be an important part of innate immune system. Aim was to evaluate expression of TLRs in peripheral blood mononuclear cells (PBMCs) during the early pregnancy in mare.Öğe Expressions of Insulin-Like Growth Factor-Binding Proteins (IGFBP) in Mare Endometrium During Estrous Cycle and Early Pregnancy.(Soc Study Reproduction, 2010) Kurar, Ercan; Atlı, Mehmet O.; Kayış, Seyit A.; Arslan, Selim; Semacan, Ahmet; Çelik, Sefa; Güzeloğlu, Aydın[Abstract not Available]Öğe Tavşanlarda Deneysel Hiperkalemi?de Salbutamol'ün Serum Potasyum Düzeyi? ve Elektrokardi?yografi?k Parametreler Üzeri?ne Etki?leri?(Selçuk Üniversitesi, 2002) Bal, Ramazan; Durgut, Ramazan; Çelik, SefaBu çalışmada materyal olarak 12 Yeni Zelanda tavşanı kullanıldı. Hayvanlar pozitif kontrol (n=6) ve salbutamol grubu (n=6) olmak üzere ikiye aynidi. Deneysel hiperkalemi oluşturmak için tavşanlara 300 mM konsantrasyondaki KCI çözeltisi (izotonik NaCl içerisinde) 0.5 ml/kg/dk hızda 15 dakika süre ile v. auricularis'den infüzyon şeklinde verildi. Salbutamol grubu tavşanlara mililitresinde 6 µg salbutamol içeren izotonik salin solüsyonu, 0.35 ml/kg/dk dozunda 30 dakika süre ile infüze edildi. Pozitif kontrol grubu tavşanlara ise 15. dakikadan sonra sadece izotonik NaCl solusyonu verildi. Tavşanların EKG çe- kimleri ilk 45 dakikalık sürede her 3 dakikada, 45. dakikadan sonra ise sadece 60 ve 90, dakikalarda yapıldı. Her iki gruba alt tavşanların ilaç verilmeyen kulağın venasından 0, 15, 30, 60 ve 90. dakikalarda kan alındı ve serum ömeklerinde potasyum analizleri yapıldı. Tavşanların deney öncesi serum potasyum düzeyi yaklaşık olarak 3.9 mmol/L olarak bulundu. Po- tasyum infüzyonu ile bu değer 6.5 mmol/L'nin üzerine çıkartıldı. Salbutamol uygulamasıyla serum potasyum düzeylerinin deney öncesi değerlerine döndüğü ve potasyumun EKG'de meydana getirdiği değişikliklerin (P dalgasında düzleşme QRS'te genişleme ve bradikardi) düzeldiği saptandı. Buna karşın pozitif kontrol grubunda ise serum potasyum düzeyindeki artışın devam ettiği ve EKG değişikliklerinin normal değerlere dönmediği belirlendi. Sonuç olarak, 32-agonisti olan salbutamolün hiperkalemi olgularında sağaltım amaçlı kullanılabileceği kanısına varıldı.
