Yazar "Şimşek, A." seçeneğine göre listele

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    Efficacies of Free and Liposome-Encapsulated Enrofloxacin (Baytril (R)) Against Staphylococcus Aureus Infection in Turkish Shepherd Dog Neutrophils in Vitro
    (Ecole National Vet Toulouse, 2000) Baş, A. L.; Elmas, M.; Şimşek, A.; Traş, B.; Yazar, E.; Hadimli, H. H.
    The influence of free and liposome-encapsulated enrofloxacin at concentrations of 1, 2 and 4 mu g/ml was studied on phagocytic and intracellular killing of Staphylococcus aureus, in cultured neutrophils of six healthy Turkish Shepherd Dogs. Enrofloxacin was encapsulated by using reverse-phase evaporation method in multilamellar liposomes composed of 15 mg egg phosphatidylcholine and 35 mg cholesterol. Phagocytic and killing activity of neutrophils were determined by fluorescent microscopic technique. Liposome-encapsulated enrofloxacin significantly enhanced the intraphagocytic killing of bacteria in neutrophils compared to free drug. Phagocytic activity of neutrophils was not significantly different among groups. The results obtained in the present study show that use of enrofloxacin encapsulated in liposomes may improve the antibacterial efficacy of the antibiotic.
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    Isolation of Bovine Herpesvirus Type 1 From Bovine Semen in Turkey
    (ECOLE NATIONALE VETERINAIRE TOULOUSE, 2001) Yavru, S.; Öztürk, F.; Şimşek, A.; Yapkıç, O.; Yıldız, C.
    In this study, semen samples from sixty bulls were used for virus isolation into sensitive cell culture. Five viruses were isolated by inoculation of 60 semen samples into the cell cultures. The isolated viruses were identified as BHV-1 according to results of cross microneutralization test by using hyperimmun sera from rabbits. Obtained data showed that BHV-1 may exist in the semen, and sensitive cattle may be infected by virus via insemination.
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    Serum tumor necrosis factor- alpha activity in dairy cows with abomasal displacement: The potential for anti-cytokine therapy
    (2004) Avki, S.; Şimşek, A.; Türütoğlu, H.; Büyükoğlu, T. İleri; Yiğitarslan, K.; Temizsoylu, M. D.
    Abomasum deplasmanlı (AD) sütçü ineklerde, serum tümör nekrozis faktör-a (TNF-a) aktivitesinin varlığını ve bu sitokin ile karaciğer hasarı arasındaki ilişkiyi araştırmak amacıyla, abomasumun sola deplasmanı (LDA) bulunan 12, abomasumun sağa deplasmanı (RDA) bulunan 4, abomasumun sağa deplasmanı ve volvulusu (RD&VA) bulunan l inek ile kontrol grubu olarak 7 sağlıklı inek incelendi. AD'lı ineklerde serum TNF- alph aktivitesi (57.13 8.10 ng/ml), kontrol ineklerine (0.08 0.01 ng/ml) göre belirgin olarak yüksek bulundu. Serum aspartat aminotransferaz (AST) (146.11 13.03 IU/1), alkalen fosfataz (ALP) (179.29 15.42 IU/1), gama-glutamil transpeptidaz (GGT) (92.19 5.07 IU/1) ve yüksek dansiditeli lipoprotein (HDL) (72.18 4.92 mg/dl) düzeyleri de kontrol grubundaki ineklerden an-lamlı oranda yüksek bulundu. Hepatosit hasarını ortaya koymak için seçilen metabolik parametrelerden, sadece serum GGT (p0.01, r 0.455) ile serum ALB konsan-trasyonlarının (p0.01, r - 0.455) TNF-a aktivitesi ile ilişkili olduğu ancak bu ilişkinin zayıf olduğu belirlendi. Elde edilen sonuçlar, AD'lı sütçü ineklerde serum TNF- alpha aktivitesinin yükseldiğini gösterdi.