Yazar "Abdallah, Hassan Hadi" seçeneğine göre listele

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    Lotus aegaeus (Gris.) Boiss and lberis sempervirens L.: Chemical fingerprints, antioxidant potential, and inhibition activities and docking on key enzymes linked to global health problems
    (ELSEVIER SCIENCE BV, 2018) Mahomoodally, Mohamad Fawzi; Vlaisavljevic, Sanja; Berezni, Sanja; Abdallah, Hassan Hadi; Zengin, Gökhan; Atanasov, Atanas G.; Mollica, Adriano
    Natural products are gaining much momentum worldwide because they have a broad spectrum of biological effects and present excellent opportunities for bioproducts development. With this fact, this study has been designed to investigate for the first time the effects of the ethyl acetate (EA), methanolic (MeOH), and water (W) extracts of Lotus aegaeus (La) and Iberis sempervirens (Is) on key enzymes as well as to quantify antioxidant capacities. The total phenolic and flavonoid content were determined using colorimetric methods and the individual phenolic compounds were assessed by LC-MS/MS analysis. Quercetin hexoside was the dominant compound in L. aegaeus, particularly in methanol extract, while the ethyl acetate and methanol extracts of I. sempervirens were characterized by the presence scopoletin and hinic acid. The antioxidant abilities of the investigated extracts were tested using different assays including free radical scavenging, reducing power, phosphomolybdenum, and metal chelating. All the extracts showed strong antioxidant abilities. For both species, the ethyl acetate extracts were more potent against cholinesterases and alpha-amylase, while the methanol extracts were most active against a-glucosidase. Only the water extract, followed by methanol of L sempervirens exhibited notable inhibitory activity against tyrosinase. In addition, the dominant compounds were docked against tyrosinase and a-glucosidase to investigate their predicted binding affinity and interactions with the active site. In conclusion, L. aegaeus and I. sempervirens showed potent biological attributes, which advocates for further studies to explore their potential use as phytopharmaceuticals.
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    Phytochemical profiling, in vitro biological properties and in silico studies on Caragana ambigua stocks (Fabaceae): A comprehensive approach
    (ELSEVIER SCIENCE BV, 2019) Khan, Saima; Nazir, Mamona; Raiz, Naheed; Saleem, Muhammad; Zengin, Gökhan; Fazal, Gazala; Saleem, Hammad; Mukhtar, Mahreen; Tousif, Muhammad Imran; Tareen, Rasool Baksh; Abdallah, Hassan Hadi; Mahomoodally, Fawzi Mohamed
    Plant from Caragana genus have interesting potential in folklore medicines and have been explored for various pharmacological activities. The aim of this study was to probe into the chemical and biological effects of different extracts of Caragana ambigua Stocks (Fabaceae). Total phenolic and total flavonoids contents were determined using standard spectrophotometric methods, whereas, the secondary metabolites composition was established by ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) analysis. Antioxidant potential was estimated via 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), phosphomolybdenum and metal chelating assays and the enzyme (cholinesterases, alpha-amylase, alpha-glucosidase and tyrosinase) inhibition potential were also assessed. Moreover, in silico docking studies were performed to highlight possible interactions between three major secondary metabolites and the tested enzymes. The ethyl acetate extract exhibited higher phenolic (85.87 +/- 2.96 mg GAE/g) and flavonoid (66.45 +/- 0.37 mg RE/g) contents, which, we propose, are responsible for its higher radical scavenging, reducing power, total antioxidant capacity and tyrosinase inhibition. The n-hexane extract showed stronger anti-cholinesterase and anti-diabetic property. Similarly, UHPLC-MS profiling of methanol and ethyl acetate extracts identified the presence of flavonoids, phenolics, alkaloids, and coumarin derivatives. Three of the dominant compounds (isobergaptene, jujubasaponin IV and phellodensin D), were docked against all enzymes and their affinity were compared. Among these compounds, phellodensin D showed highest affinity towards all the studied enzymes. It is therefore, concluded that extracts from C. ambigua showed potent antioxidant and enzyme inhibition potential with potent bioactive molecules which could open new industrial applications.