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    Comparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochlorite
    (Dental Investigations Society, 2015) Ok E.; Adanir N.; Hakki S.
    Objective: The purpose of this in vitro study was to compare the cytotoxicity of 0.5-4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts. Materials and Methods: About 0.5-4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fibroblasts. Viability of hPDL cells was evaluated with WST-1 (Cell Proliferation Reagent WST-1 Roche) test at 1, 24 and 72nd h. hPDL cells were plated at 20 × 103 cells per well in 96-well plates. Absorbance values were read in optical density 480 nm by ELISA plate reader spectrophotometer. The statistical differences between various groups were evaluated using one-way ANOVA, post-hoc Duncan's Multiple Range test using SAS software. Statistically, a significant difference was considered at P < 0.001. Results: According to the 1-h cytotoxicity results, 0.5% OES showed the least cytotoxic effect in test groups. There were not found any statistical significance between 1% OES and 2% CHX. About 5.25% NaOCl showed more cytotoxic effect than 1% OES and 2% CHX. In 24 and 72 h, different concentrations of OES, 5.25% NaOCl, 2% CHX solutions showed similar cytotoxic effect. Conclusions: Based on these results, 1% OES and 2% CHX showed similar results and less cytotoxic effect than 5.25% NaOCl. It could be considered as a favorable solution concentration when OES was used as root canal irrigation solution. © 2015 Dental Investigations Society.
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    In vitro evaluation of the dissolving effect of solvents on root canal sealers.
    (2003) Erdemir A.; Adanir N.; Belli S.
    The purpose of this in vitro study was to evaluate two commonly used gutta-percha solvents for their effectiveness in dissolving several types of root canal sealers. Seven different sealers (AH26, AH Plus, Diaket, Roekoseal, Sankin Apatite Root Sealer, Sealapex, and Sultan U/P) were used in this study. After mixing according to the manufacturers' directions, each material was syringed into 30 glass capillary tubes, and a total of 210 tubes were placed in a humidifier at 37 degrees C for one week to allow the materials to set completely. Each group of 30 tubes, obturated with one type of sealer, was then randomly divided into three subgroups, including 10 tubes each. Chloroform was used in the first ten tubes from each sealer group. Halothane was used for the second group. In the last group, the sealer was removed with files, without using any solvent. The time necessary to pass a file through to the end of the tube was recorded for each sample in seconds. Results were analyzed using one-way analysis of variance. Sealapex did not set at all unless in contact with air. Roekoseal did not adhere to the glass capillary tubes, and was therefore easily removed from the tube in all samples. AH26 and AH Plus root canal sealers tightly adhered to the tube walls, so none of the techniques were effective in removing them from the tubes within 30 min. Diaket root canal sealer was easily removed using solvents (P < 0.05). There was no advantage in using solvents to remove Sankin Apatite Root Sealer (P > 0.05). Solvents were found to be very effective in dissolving the Sultan U/P root canal sealer (P < 0.05).