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  • Küçük Resim Yok
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    Cytokines, adipocytokines and inflammatory markers in patients on continuous ambulatory peritoneal dialysis and hemodialysis
    (TAYLOR & FRANCIS LTD, 2016) Oncel, Mufide; Akbulut, Seval; Ozer, Turkan Toka; Kiyici, Aysel; Keles, Mustafa; Baltaci, Beyhan; Turk, Suleyman
    Background: Cytokines are essential mediators of immune response. Chronic renal failure patients suffer from chronic inflammation that results from factors such as impaired renal function, accumulation of uremic toxins and bio incompatibility of dialyzer membranes. These patients are also at increased risk of cardiovascular diseases. We have evaluated cytokines, adipocytokines and inflammatory markers in patients with chronic renal failure undergoing hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD).Material and methods: We have determined serum tumor necrosis factor- (TNF-), interleukin-6 (IL-6), high-sensitivity C-reactive protein (hsCRP), leptin and ghrelin levels of chronic renal failure patients treated with either HD (n=20) or CAPD (n=20). TNF-, IL-6, ghrelin and leptin measurements were performed by commercially available kits based on enzyme-linked immunosorbent assay (ELISA) method. hsCRP levels were determined by turbidimetric methods.Results: Serum TNF- and IL-6 levels of patients on HD were significantly higher than those of the ones on CAPD (p<0.05). Ghrelin, leptin and hsCRP concentrations were similar in both groups.Conclusions: We can conclude that cytokine production is more obvious in HD process.
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    Deneysel nekrotizan enterokolit modelinde ağız yolu ile verilen immünglobülin A'nın etkisi
    (2008) Aydoğdu, Bahattin; Yurtçu, Müslim; Akbulut, Seval; Gürbilek, Mehmet; Toy, Hatice; Günel, Engin
    Amaç: Deneysel/olarak oluşturulan nekrotizan enterokolit (NEK) modelinde, oral yoldan verilen immünglobülin A'nın sıçan barsağını koruyucu etkisini araştırmayı amaçladık. Gereç ve Yöntem: 40 yenidoğan sıçan 10'arlı gruplar halinde 4 gruba ayrıldı. Kontrol (K) grubu anne yanında bırakılırken, NEK (N), sham (S) ve tedavi (T) grubu anne sütü almadan, annesinden ayrı olarak 36C de ve % 60'lık nemde beslenme ve bakım sağlanmak üzere inkübatöre yerleştirildi. K grubundaki sıçanlar anne sütü ile beslendi. N grubundaki denekler doğar doğmaz, hiç anne sütü almadan annelerinin yanından ayrılarak formula mama ile beslendi. T grubundaki sıçanlara hiç anne sütü almadan formula mamaya ilaveten 600 mglkglgün 6 doz halinde saf oral IgA verildi. S grubundaki deneklere hiç anne sütü almadan mamaya ilaveten 0.1 mllkglgün immunoglobulin çözücüsü olan distile su verildi. Tüm gruplardaki sıçanlar 4. gün tartılarak sakrifiye edildi. Laparatomi sonrası ile-oçekal valvin 1 cm proksimalinden 2 cm'lik barsak segmenti histopatolojik inceleme için, geri kalan 10 cm'lik segment biyokimyasal inceleme için çıkartıldı. H&E boyama ile histopatolojik, ARC (Apoptosis Repressor With CARD) Ab-1 apopitozis kiti kullanılarak immunohistokimyasal, doku Myeloperooksidaz (MPO), Tümör nekrozis faktör alfa (TNF-a), ve İnterlökin altı (IL-6) bakılarak biyokimyasal değerlendirme yapıldı. Bulgular: N ve S gruplarında mortalite oranı T ve K gruplarına göre anlamlı olarak yüksek bulundu (P0.05). K grubunda istatistiksel olarak anlamlı% ağırlık artış olduğu tespit edilirken, diğer gruplarda ağırlık azalması tespit edildi (P0.05). Histopatolojik değerlendirme ve apopitozis dağılımına bakıldığında, T grubunun, N ve S grubundan anlamlı azalma olduğu görüldü (P0.05). Doku IL-6, TNF-a ve MPO seviyeleri incelendiğinde, T grubunun S ve N gruplarından anlamlı olarak düşük olduğu (P0.05), ayrıca T ile K grubu arasında anlamlı fark olmadığı tespit edildi (P0.05). Sonuç: Oral yolla verilen saf lgA'nın deneysel NEK modelinde, intestinal hasan azalttığı ve NEK' ı önlediği görüldü.
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    Diabetik polinöropatili hastalarda eritropoietin uygulamasının eritrosit Na+-K+ ATPaz enzim (E.C.3.1.6.37) aktivitesi üzerine etkilerinin araştırılması
    (Selçuk Üniversitesi Tıp Fakültesi, 2008) Akbulut, Seval; Gürbilek, Mehmet
    Diabetik polinöropatili hastalarda eritropoietin uygulamasının Na+-K+ ATPaz enzim (E.C.3.1.6.37) aktivitesi üzerine etkilerinin araştırılması için yapılan bu çalışma 48-72 (60 ± 8,5) yaşları arasında, EMG ile polinöropati teşhisi konulmuş ve EPO tedavisine başlanacak toplam 11 diabetik polinöropatili hasta (3 erkek, 8 kadın) ile 48-56 (51,3 ± 3,05) yaşları arasında tamamen sağlıklı 10 kontrol (5 erkek, 5 kadın) vakası üzerine gerçekleştirildi. Hastalardan EPO tedavisi öncesi ve tedavinin 30'uncu günlerinde ve kontrol grubundan da bir kez heparinize kan örnekleri alındı ve örneklerden eritrosit membranı izole edilerek eritrosit membranı Na+- K+ ATPaz enzim aktivitesi ölçümü yapıldı. Enzim aktivitesi modifiye edilmiş Kitao-Hattori metodu ile ölçüldü. Na+- K+ ATPaz enzim aktiviteleri sırasıyla kontrol grubu, tedavi öncesi hasta grubu ve tedavi 30'uncu gün hasta grubunda 2,91±0,38 ? molPi.mg.prt?1.10dak-1, 1,93±0,38 ? molPi.mg.prt?1.10dak-1 ve 2,40±0,63 ? molPi.mg.prt?1.10dak-1 olarak ölçüldü. Na+- K+ ATPaz enzim aktivitesi düzeyi, tedavi öncesi hasta grubunda kontrol grubuna göre anlamlı olarak düşük, Epo tedavisinden sonra 30'uncu günde tedavi öncesine göre anlamlı olarak yüksek bulundu. Na+- K+ ATPaz enzim aktivitesi düzeyinin Epo tedavisinden sonra yükseldiği ve 30'uncu günde hasta grubu ile kontrol grubu arasında anlamlı bir fark olmadığı görüldü. EPO tedavisinin nöropatiyi iyileştirebildiği ve bu iyileştirmenin, belirteç olarak kullanılabilen Na+-K+ ATPaz enzim aktivitesi artışı ile desteklendiği sonucuna varılmıştır.
  • Küçük Resim Yok
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    Effect of oral immunoglobulin A in experimental necrotisan enterocolitis model
    (2008) Aydoğdu, Bülent; Yurtçu, Müslim; Akbulut, Seval; Gürbilek, Mehmet; Toy, Hatice; Günel, Engin
    Aim: Investigation of the protective effect of oral immunglobulin(Ig) A on rat intestinum in experimental necrotising enterocolitis (NEC) model. Materials and methods: 40 newborn rats were divided into 4 groups each containing 10 rats. While control (C) group was fed by breast, the rats in necrotisinge enterocolitis (N), sham (S), and treatment (T) groups were settled into incubators at 36°C and 60 % humidity and fed, but not by breast. The rats in C group were fed by breast. The rats in N group were fed with Formula as soon as they were born. The rats in T group were fed with Formula and 600 mg/kg/day oral Ig A with 4-hour intervals. The rats in S group were fed with Formula and 0.1 ml/kg/day distilled water which is solvent of Ig. The rats in all groups were weighed and sacrified on fourth day. 2 cm intestinal segment from proximal of ileocaecal valve was used for histopathologic examination, another 10 cm intestinal segment for biochemical examination. After laparotomy, H&E was used for histopathological examination and apoptosis repressor with card Ab-l citt for immunohistochemical examination. Biochemical parameters such as myeloperoxidase (MPO), TNF-?, and IL-6 were evaluated. Results: The rate of mortality in N and S groups was significantly higher than T and C groups (P<0.05). Significant weight increase was identified in C group (P<0.05) There was significant decrease in T group in comparison of histopathologic values and apoptosis according to N and S groups (P<0.05). T group was significantly different in comparison of IL-6, TNF-?, and MPO according to S and N groups (P<0.05). There was no significant difference between T and C groups (P>0.05). Conclusion: Pure IgA given orally was identified to decrease intestinal damage and to prevent NEC in experimental NEC model.
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    Investigation of Serum Crosslinked N-Telopeptides of Type I Collagen (Ntx) Levels and Total Antioxidant Capacity in Patients with Type 2 Diabetes Mellitus
    (Walter De Gruyter Gmbh, 2010) Kurban, Sevil; Mehmetoğlu, İdris; Gönen, Sait; Akbulut, Seval
    Purpose: Our aim was to investigate the serum cross-linked N-telopeptides of type I collagen levels and total antioxidant capacity in patients with type 2 diabetes mellitus. Methods: 46 patients with type 2 diabetes mellitus (24M, 22F) aged 41 to 68 years (54.36 +/- 7.92) and 34 healthy controls (17M, 17F) aged 40 to 69 years (52.91 +/- 8.04) were enrolled in the study. Serum cross-linked N-telopeptides of type I collagen levels were measured by enzyme-linked immunosorbent assay method using a commertially available kit, total antioxidant capacity levels were measured by a colorimetric method based on the oxidation of 2,2'-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid) radical and hemoglobin A(1c) levels were measured by high performance liquid chromatography technique. Results and Conclusion: Serum cross-linked N-telopeptides of type I collagen levels of the patients were significantly higher (p<0.01) whereas total antioxidant capacity levels were significantly lower (p<0.05) than those of the controls. Also, there was a significant positive correlation between serum cross-linked N-telopeptides of type I collagen and hemoglobin A(1c) levels (r=0.301, p<0.05) and a significant negative correlation between total antioxidant capacity and hemoglobin A(1c) levels (r=-0.382, p<0.01) in the patients group. In conclusion, our results show patients with type 2 diabetes mellitus have a significantly reduced total antioxidant capacity levels and there is a risk of bone resorption in these patients which can be estimated by measuring serum cross-linked N-telopeptides of type I collagen levels.
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    Serum TNF- Alpha Levels in Acute and Chronic Pancreatitis
    (2009) Kıyıcı, Aysel; İbiş, Mehmet; Akbulut, Seval; Köklü, Seyfettin; Uçar, Engin; Ünlü, Ali
    Aim: Acute and chronic pancreatitis are inflammations of pancreatic tissue which have systemic effects and clinical presentations such as bacteremia and septic shock. Inflammatory markers have growing importance in diagnosis and identification of the severity of the pancreatitis. We aimed to determine TNF-alpha levels in acute and chronic pancreatitis and evaluate the relation between TNF-alpha levels and the pancreatic enzyme concentrations in two forms of the disease. Methods: 13 patients with acute pancreatitis, 36 patients with chronic pancreatitis and 14 healthy controls were included to our study. TNF-alpha determinations were performed with ELISA method. Results: TNF-alpha concentrations were 13.30±4.42 (7.04-21.35) pg/ml and 9.88±4.68 (3.99-27.73) pg/ml 10.09±1.01 (8.69-14.96) pg/ml in patients with acute and chronic pancreatitis and healthy controls respectively. TNF-alpha concentrations were significantly higher in patients with acute pancreatitis than the patients with chronic pancreatitis. But there was no significant difference between healthy controls and patients with either acute or chronic pancreatitis for TNF-alpha levels. There was no significant correlation between TNF-alpha concentrations and pancreatic enzyme levels. Conclusion: We concluded that in acute pancreatitis TNF-alpha levels were higher than the chronic form of the disease. But its concentrations did not correlate with the severity of the disease. By investigation of the other inflammatory markers and acute phase reactants with TNF-alpha, this process will be more clarified.