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Öğe Autologous Transplantation of Arterial Cells Improves Cardiac Function in a Rabbit Model of Infarcted Myocardium(Mary Ann Liebert, Inc, 2010) Tokaç, Mehmet; Aktan, Murad; Ak, Ahmet; Duman, Selçuk; Tokgözoğlu, Lale; Aygül, Nazif; Paksoy, Yahya; Porat, YaelCellular cardiomyoplasty is a promising approach for the treatment of severe heart failure. However, the question which cell line is the best to use is still a matter of debate. In this study, we aimed to evaluate the efficacy of arterial media-intima cell suspension (AMICS) transplantation in rabbit myocardial infarct model. The study was divided into 2 groups: group A (the cell-treated group, n = 9) and group B (the medium injection group, n = 8). Group A was further divided into 2 subgroups as branch-1 (treated with unlabeled cells) and branch-2 (treated with iron-labeled cells). The experimental myocardial infarction (MI) was induced by ligation of left anterior descending coronary artery with a combination of cryoinjury. Ten days after the MI, cells obtained from autologous femoral arteries were injected into the injured myocardium of group A, while group B received an injection of only DMEM medium. Clinical, echocardiographic, and histopathologic evaluations were done. As compared to the ninth day values, echocardiography showed a significant improvement in systolic functions and left ventricular (LV) dimensions of the cell-treated group on the 30th day. In the heart biopsy sections of branch-1, the immunostained injected cells were observed to exist closely, suggesting an organization. Cells existing separately and lumen-like structure organizations stained positive with both smooth muscle cell (SMC) a-actin and Prussian Blue were also showed in the histological observation of branch-2. Autologous AMICS transplantation seems to be a feasible and efficacious method for cellular cardiomyoplasty in our rabbit model.Öğe Comparison of pregnancy outcomes of progesterone or progesterone plus estradiol for luteal phase support in ICSI-ET cycles(KARGER, 2004) Görkemli, Hüseyin; Ak, Dilek; Akyürek, Cemalettin; Aktan, Murad; Duman, SelçukObjective: To find out the effect of estradiol with progesterone for luteal phase support in IVF-ICSI cycles. Materials and Methods: Patients were accepted for treatment in the ART unit of Selcuk University, Meram Faculty of Medicine, between January 2001 and March 2003. The study was done in a prospective manner. The age range of 252 women was 19 - 41 years and the total number of cycles was 310. All patients were treated with a long ovulation induction protocol. Patients were treated and divided into two groups in a randomized manner: group I used only 600 mg/day divided into three equal doses of micronized progesterone vaginally, and group II used transdermal estradiol 100 mug/day + 600 mg/day vaginal micronized progesterone. Results: 310 ICSI cycles were carried out in 252 infertile couples between January 2001 and March 2003. From 22 of these cycles, oocytes were retrieved but no embryos were developed. In the remaining 288 cycles there were embryo transfers. All embryo development was achieved by ICSI treatment. In 148 out of 288 cycles, the luteal phase was supported only by vaginal micronized progesterone ( group I). On the other hand, the remaining 140 cycles received vaginal micronized progesterone plus transdermal estradiol 100 mug/day ( group II). The number of beta-hCG-positive results in group I and group II were 20 (13.5 %) and 54 (38.5%) respectively. Conclusion: Adding estradiol to progesterone for luteal phase support in ICSI-ET cycles may increase implantation and pregnancy rates. Copyright (C) 2004 S. Karger AG, Basel.Öğe Effects of ovulation induction on ovarian morphology: an animal study(BLACKWELL PUBLISHING INC, 2004) Çelik, Çetin; Gezginç, Kazım; Aktan, Murad; Acar, A.; Yaman, Süleyman; Güngör, S.; Akyürek, CemalettinObjective: The aim of this study was to investigate whether the ovulation induction has relation with postneoplastic lesions. Materials and methods: Seventy-eight female, 90-day-old rats were enrolled for the trial. They were divided into three groups. In the first group, 13 rats received one cycle of ovulation induction with Follitropin Beta and human chorionic gonadotropin. The second group of 13 rats received three cycles of ovulation induction, and the third study group consisted of 13 rats which received six cycles of ovulation induction. Each group had a control group consisting of same number of rats that had not received ovulation induction. At the 12th month after the ovulation induction protocols ended, rat ovaries were extirpated for histopathological examination. In histopathological examination, malignant lesions, ovarian cyst and cyst diameter, epithelial stratification, epithelial tufting, mitotic index, polymorphism of epithelial cells and nucleus, epithelial cell nuclear diameter, chromatin density nuclear atypia, and mitotic activity in ovarian cyst epithelium were evaluated. Results: No malignant ovarian lesion was found in the three groups. Ovarian cyst development was most frequent in the rats that underwent six cycles of ovulation induction. Epithelial stratification and tufting were most frequent in the rats which underwent ovulation induction six times. Significant difference was found between induction and control groups in second and third groups for cellular and nuclear polymorphism, presence of nucleolus, and nuclear chromatin density. Conclusions: Although development of malignant lesion were not found in any of the rat ovaries after ovulation induction, increase in the prevalence of epithelial dysplasia especially with increase in the number of induction cycles shows that some ovarian pathologies can occur subsequent to ovulation induction.Öğe Ginkgo Biloba Eksterinin Dondurulmuş-Çözülmüş Sıçan Kornea Epitel Hücrelerinin Viabilitesine Etkisi(2002) Okka, Mehmet; Aktan, MuradDoku organizasyonundan hücre süspansiyonu haline getirilen kornea epitel hücrelerinin dondurup-saklama sonrası viabilite oranına ginkgo biloba ekstresi (EGb 761)'nin etkisinin araştırılması. Yöntem: Wistar albino tipinde 7 adet erkek sıçanın korneaları 360 limbal insizyon ile eksize edildi. Korneaların epitel tabakaları hücre süspansiyonu haline getirildi. Epitel hücreleri homojenize edilerek iki adet tüpe dağıtıldı. Tüplerden bir tanesine EGb 761 ilave edilerek hızlı bir dondurma işlemi uygulandı. Dondurma işlemi öncesi ve dondurulup saklandıktan sonra ısıtılmayı takiben Eosin-Y ile viabilite ölçümü yapıldı. Bulgular: Dondurma işlemi öncesi Eosin- Y ile yapılan viabilite ölçümlerinde kontrol grubu viabilite ortalaması % 45.5, EGb 761 grubu viabilitesi ortalaması % 37.5 bulunmuştur. Bu iki grup arasında viabilite açısından anlamlı bir fark bulunmamıştır (P 0.061). Isıtıp çözme işlemini takiben viabilite ölçümü ortalamaları ise kontrol grubunda % 15.8, EGb 761 grubunda ise % 31.08 olarak bulunmuştur. İki grup arasında istatistiksel olarak anlamlı bir fark olduğu görülmüştür (P 0.0002). Sonuç: EGb 761 ilavesi sıçan kornea epitel süspansiyonunun dondurulup-saklanmasında koruyucu bir etki göstermektedir.Öğe I?nsan Erkek Gonadlarının Fetal Dönemdeki Gelişiminin Histolojik Değerlendirilmesi(2001) Tosun, Murat; Büyükmumcu, Mustafa; Aktan, Murad; Duman, Selçuk; Kalkan, Serpil; Soylu, RefikAmaç: Selçuk Üniversitesi Tıp Fakültesi Histoloji Embriyoloji Anabilim Dalında yapılan bu çalışmada fetal dönemde erkek gonadlarının gelişiminin izlenmesi amaçlanmıştır. Materyel ve Metod: Selçuk Üniversitesi Tıp Fakültesi Anatomi Anabilim Dalında spontan düşük yoluyla elde edilmiş 26 adet erkek fetüs piyesinin gonadları çıkartıldıktan sonra klasik parafin tekniği ile takip edilip bloklandı. Her blokdan 5 m kalınlığında kesitler alınıp Hematoksilen Eosin ile boyandı. Kesitler ışık mikroskobunda değerlendirildi. Bulgular: Seminifer tübüllerin fetal gelişim sürecinde ilk haftalarda çok değişik çap ve büyüklüklerde olduğu ancak ilerleyen haftalarda benzer yapısal özellikler gösterdiği görüldü. Seminifer tübüller içinde yer alan spermatogoniaların ve Sertoli hücrelerinin sayılarının giderek arttığı, bununla birlikte, Leydig hücre sayısının 19. haftaya kadar arttığı ve bu dönemden sonra azaldıkları tespit edildi. Seminifer tübül lümeninin 19. hafta civarına kadar mevcut olmadığı, interstisiyel dokunun yani ekstrakordal bölümün ilerleyen yaşa bağlı olarak daha düzenli ve organize bir yapı aldıkları tespit edildi. Sonuç: Elde edilen bulgular gonadların intraembriyonik gelişimlerinin çok kompleks ve bununla birlikte sistematik bir düzen içinde gerçekleştiğini ortaya koymaktadır.Öğe I?ntrauterin Dönemde Dişi ve Erkek Gonadal Yapıların Gelişimine Etkin Olan Faktörler(2000) Tosun, Murat; Aktan, Murad; Duman, Selçuk; Erdoğan, Ender; Taşkapu, Hakanİnsanlarda dişi ve erkek gonadların intrauterin dönemdeki gelişimini birçok faktör doğrudan veya dolaylı yoldan etkilemektedir. Bunların içinde en önemli olanları başta genetik olmak üzere endokrinolojik ve çevresel faktörlerdir. Genetik faktörler içinde H-Y antijeni, WT-1 geni, Sry geni gibi çok önemli genler bulunurken; endokrinolojik faktörler içinde cinsiyet farklılaşmasında çok öneme sahip Anti Müllerian Hormon (AMHMüllerian Inhibiting SubstanceMIS)’un yanında Follikül Stimulan Hormon (FSH), Luteinizan Hormon (LH) ve Serotonin yer almaktadır. Bunların dışında Fibroblast Growth Faktör (FGF), Aromataz, Aktivin, İyonize radyasyon ve Mitomisin C gibi değişik çevresel ve kimyasal faktörler de gonadal gelişimi etkilemektedir. Bu veriler bize gonadların intrauterin gelişiminin oldukça kompleks olduğunu ve sayısız faktörün bu gelişimi doğrudan veya dolaylı yoldan olumlu veya olumsuz yönde etkilediğini ortaya koymaktadır.Öğe Improvement in rabbit corneal cell suspension viability after freezing with Gingko Biloba extract.(2007) Aktan, Murad; Okka, Berrin; Okka, Mehmet; Duman, SelçukWe investigated whether the addition of Gingko Biloba extract (EGb 761) to rabbit corneal epithelial medium before cell freezing improved cell viability after freezing then thawing. After removal of corneas, they were treated with enzymes and the corneal epithelium was prepared as a single cell suspension in freezing media with or without EGb 761. After freezing for two weeks then thawing, a higher cell viability was found in the cornea cell suspensions which had been frozen pretreated with EGb 761 in the media. The improvement with corneal cell viability with EGb 761 pretreatment is postulated to be based on the antioxidant capacity of the plant extract.Öğe Influence of cumulus cell coculture and cumulus-aided embryo transfer on embryonic development and pregnancy rates(AVES, 2010) Cihangir, Nalan; Görkemli, Hüseyin; Özdemir, Suna; Aktan, Murad; Duman, SelçukObjective: In this study, we aimed to evaluate the influence of autologous cumulus oocyte complex (COC) coculture on embryonic development and quality, and investigate the implantation and pregnancy rates after cumulus-aided embryo transfer in the ICSI-ET cycles. Material and Methods: Ninety five consecutive infertile women undergoing their first cycle of IVF teratment were included in the study. The cases were divided into two groups. Group 1 consisted of 48 women undergoing ICSI, along with autologous cumulus embryo coculture and cumulus-aided emryo transfer. Group 2 comprised 47 consecutive patients who consented to undergo ICSI and in whom autologous cumulus embryo coculture and cumulus-aided embryo transfer were not performed. Implantation and pregnancy rates were compared between the two groups. Results: The demographic data and controlled ovarian hyperstimulation parameters were similar in the two groups. The fertilization and cleavage rates were found to be higher in group 1 when compared with group 2 (p=0.03 and 0.001, respectively). There were no statistical significant differences for the implantation and clinical pregnancy rates between the two groups. Conclusion: Usage of autologous COCs as coculture may improve fertilization and cleavage rates. However, cumulus-aided embryo transfer does not produce an increase in implantation and pregnancy rates.Öğe Morphometric analyses of the development of dermis in human fetuses(1999) Aktan, Murad; Büyükmumcu, Mustafa; Şeker, Muzaffer; Mermer, S.; Duman, SelçukIn this study, the morphometric differences of the different derrnis regions during the development of the human fetus were investigated. The different dermis regions, namely, cranial, femoral and plantar (sole) parts of 40 fetuses which were between 12th-23.5th week gestation age were obtained from maternity hospital and after the routine H&E histological procedure, the measurement was performed with ocular micrometer. The first sharp increase was observed in the dermis of cranial region. It has been suggested that the thickness of the dermis is dependent on the collagen and elastic fibres, but the flexibility of the dermis is dependent on the number and the structure of the elastic fibres. The early beginning of the increase of the dermis thickness in the cranial part may be due to the effect of the cranio-caudal stimulation on the production of the collagen and elastic fibres in the embryonic term.Öğe Protective effects of growth hormone on bacterial translocation and intestinal damage in rats with partial intestinal obstruction(COMENIUS UNIV, 2014) Kaymakcı, Aytekin; Güven, Şirin; Çiftçi, İlhan; Akıllıoğlu, İshak; Aktan, Murad; Eker, Hasan Hüseyin; Sütçü, A.Objective: One of the reasons of bacterial translocation (BT) is the complete or partial intestinal obstructions (PIO) of the gastrointestinal system. In this study, we aimed to investigate the effects of recombinant human Growth Hormone (rhGH) on BT in rats with partial intestinal obstruction (PIO). Material and methods: The rats were randomly divided into the 4 groups: Group I: Sham-operated (SO) (n = 12), Group II control PIO (n = 12), Group III: PIO with rhGH treatment for 5 days (n = 12), Group IV: PIO with rhGH treatment 5 days before PIO and 5 days after PIO (a total of 10 days) (n = 12). In the groups III and IV, the effects of 5 and 10 days administered rhGH were examined. Results: The level of serum and of intestinal fluid IgA was significantly higher in the Group IV compared to the Group I, Group II and Group III. In the Group IV, the number of small intestinal goblet and colonic goblet cells, and the lengths of intestinal mucosal villi and crypt depths were statistically significantly higher than in Groups II and III. The rate of bacterial translocation was higher in the Group II: 100 % in MLNs, 41.6 % in blood culture and 50.8 % in the liver cultures, it was significantly higher compared to the other groups (p < 0.01). Conclusions: The study results demonstrated that administration of rhGH to the rats with PIO for at least 10 days decreased bacterial translocation (Fig. 3, Ref. 25). Text in PDF www.elis.sk.Öğe The role of inflammation markers in triggering acute coronary events(SPRINGER, 2003) Tokaç, Mehmet; Özeren, Ali; Aktan, Murad; Altunkeser, Bülent Behlül; Özdemir, Kurtuluş; Düzenli, Akif; Gök, HasanStudies have shown disparate results in relation to the role of plasma concentrations of inflammation markers such as fibrinogen, cytokines, and cell adhesion molecules in acute coronary syndromes. The differentiation of primary versus secondary alterations of these markers in response to acute coronary syndromes is not clear. The aim of this study was to investigate the effect of soluble cell adhesion molecules and some inflammatory markers on coronary plaque instability. The prospective study consisted of 15 patients with stable angina pectoris (SAP), 16 with unstable angina pectoris (UAP), and 16 who had undergone percutaneous transluminal coronary angioplasty (PTCA). Blood samples were obtained from the SAP group on admission, from the UAP group at the early stage of pain onset within 6h of pain, and again after 12h of pain. Samples from the PTCA group were collected before, 2, 14h after the procedure. Soluble vascular cell adhesion molecule-1 (VCAM-1), endothelial selectin, interleukin-1beta (IL-1beta) and interleukin-2 (IL-2), and C-reactive protein (CRP) were analyzed by enzyme-linked immunosorbent assay. CRP serum levels gradually increased although IL-2 gradually decreased in patients with UAP and PTCA. In addition, VCAM-1 levels were sharply decreased after the PTCA procedure. However, this value returned back to the preprocedure levels 14h after PTCA. Both CRP and IL-2 are directly involved in the triggering mechanisms of acute coronary events.Öğe Sperm retrieval and IVF-ICSI results in the treatment of obstructive and non-obstructive azoospermia(2005) Yurdakul, Talat; Görkemli, Hüseyin; Aktan, Murad; Duman, Selçuk; Güven, SelçukOBJECTIVE: It is usually possible to recover sperm in both obstructive and non-obstructive azoospermic patients by microsurgical epididymal aspiration (MESA) or testicular sperm extraction (TESE) and ICSI could be performed with these spermatozoas. STUDY DESIGN: Ninty-one males were diagnosed as azoospermia in the Urology Department, when sperm was able to recovered, intracytoplasmic sperm injection (ICSI) treatment was applied in our Assisted Reproductive Technology (ART) unit during the period of November 2000 to April 2003. MESA was performed to 13 obstructive azoospermic (OA) males and TESE was performed to 78 non-obstructive azoospsermic (NOA) males. RESULTS: Mature spermatozoa were picked up from all of the obstructive azoospermic patients. Mature spermatozoa were also picked up from 47 (60%) NOA patients. 92 ovulation induction cycles were carried out for ICSI. Of these cycles 29 were carried out with epididymal mature spermatozoa and 63 cycles carried out with testicular mature spermatozoa in non-obstructive azoospermics. In MESA group, fertilization rate was 149/243 (61%). Biochemical pregnancies were achieved in 6 couples (46%), a healthy twin was delivered in one couple (7.6%) and pregnancies of two couples are ongoing. In TESE group, fertilization rate was 181/488 (37.3%). Biochemical pregnancies were achieved in 13 couples (27%) and in 7 (14.8%) couples healthy delivery were done, one of them was twin . CONCLUSION: When sperm can be picked up by MESA or TESE from an azoospermic patient,IVF/ICSI is the only choice of treatment for these infertile couples.
