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Öğe The Effects of Caffeic Acid Phenethyl Ester and Ellagic Acid on the Levels of Malondialdehyde, Reduced Glutathione and Nitric Oxide in the Lung, Liver and Kidney Tissues in Acute Diazinon Toxicity in Rats(MEDWELL ONLINE, 2011) Alp, Harun; Aytekin, Ismail; Atakisi, Onur; Hatipoglu, Namik Kemal; Basarali, Kemal; Ogun, Metin; Buyukbas, SadikThe aim of this study was to investigate the effects of Caffeic Acid Phenethyl Ester (CAPE) and Ellagic Acid (EA) on acitivities of Malondialdehyde (MDA), reduced Glutathione (GSH) and Nitric Oxide (NO) in rat lung, liver and kidney tissues in acute Diazinon (DI) toxicity. Six groups of 6 Sprague Dawley rats were used comprising control, CAPE, EA, DI control, DI+CAPE and DI+EA. Tissue samples were analysed for GSH, MDA and NO levels in lung, liver and kidney tissues. Biochemical parameters were measured colormetrically by spectrophotometer. Control, CAPE and EA groups showed no statistically significant difference whereas DI+medication groups revealed that CAPE and EA increased the level of GSH in liver tissue by blocking the DI effect. NO levels in lung, liver and kidney tissues were significantly increased by DI but CAPE and EA attenuated those levels. In DI+medication groups, MDA levels showed no significant change in kidney and liver tissues but in lung tissues, CAPE and EA reduced the MDA level by blocking the DI effect. It was concluded that CAPE and EA which showed similar effects to each other could be used for protection and support against oxidative stress caused by acute DI intoxication.Öğe Effects of caffeic acid phenethyl ester, ellagic acid, sulforaphane and curcumin on diazinon induced damage to the lungs, liver and kidneys in an acute toxicity rat model(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2011) Alp, Harun; Aytekin, Ismail; Esen, Hasan; Basarali, Kemal; Kul, SevalThe aim of this study was to investigate the possible protective effects of caffeic acid phenethyl ester (CAPE), ellagic acid (EA), sulforaphane (SFN) and curcumin (CUR) against the toxic effects of diazinon (DI). Sixty Sprague Dawley rats were randomly divided into 10 groups. Five groups were allocated as control groups comprising unmedicated control, CAPE, EA, SFN and CUR control groups. The remaining five groups were the study groups comprising DI, DI + CAPE, DI + EA, DI + SFN, and DI + CUR groups. The animals were sacrified 24 h after drug administrations. DI caused a decrease in acetyl cholinesterase (AChE) activity (P<0.05) and increases in gamma-glutamyltransferase (GGT) and amylase activities. It also damaged the kidney, liver, and lung tissues. The negative effects of DI on these enzymes were confirmed histopathologically. Also, CAPE, EA, SFN and CUR reduced amylase and GGT activities and caused an increase in the AChE activities that were increased due to the toxic effects of DI. Thus, it was determined biochemically and histopathologically that these medication reduced the degenerative toxic effects created by DI in the lung, liver and kidney tissues. These findings led us to believe that CAPE, EA, SFN and CUR may be used as protective medicines in acute DI intoxication.Öğe Effects of exercise and caffeic acid phenethyl ester after chronic exercise rat model(JOURNAL SPORTS SCIENCE & MEDICINE, 2011) Alp, Ayşe; Büyükbaş, Sadık; Alp, Harun; Gergerlioğlu, H. Serdar; Öz, Mehmet; Başarali, M. Kemal; Kıyıcı, AyselIn order to understand whether exercise and caffeic acid phenethyl ester (CAPE) has an effect on obesity and weight control, we investigated the effects of CAPE, and exercise on lipid parameters (triglyceride, total cholesterol, HDL-C, LDLC), and adipokine substances such as leptin and resistin in rats. 40 male rat were randomly assigned into 4 groups. It was determined that CAPE does not have any significant effect on these parameters but that lipid parameters and leptin values in exercise groups decreased considerably, while no significant change occurred in resistin levels. In order to understand whether diet has an effect on exercise, body weights of all animal groups in pre and post-exercise were compared. A significant weight gain was observed (p = 0.005) in all groups. This study concluded that exercise has a considerable effect on leptin and lipid parameters; however, exercise alone was not sufficient for weight control and could be effective in weight control only when accompanied by a restricted diet.Öğe Metforminin Melanom Cilt Kanseri Hücreleri Üzerindeki Etkisinin Araştırılması(Selçuk Üniversitesi, 2021) Alp, Harun; Tutun,Hidayet; Altıntaş,Levent; Kaplan, Halil Mahir; Şingirik, Ergin; Kahraman, İbrahimAmaç: Kanserlerde kemoterapötiklere gösterilen dirençte ve kanser gelişiminde apoptozisin önemli rolü vardır. Metformin tip 2 diyabet tedavisinde kullanılan güvenli, ucuz ve etkili bir ilaçtır. Ayrıca, antikanser etkilerinin olduğuna dair kanıtlar olmasına rağmen metforminin bu etkiyi hangi mekanizmalar ile gerçekleştirdiği açık değildir. Bu nedenle, bu çalışmada metformin cilt kanseri hücrelerinde Bcl-2, caspaz-3, bax, wee-1, AIF, grp78 ve gadd153 düzeyleri üzerine etkileri araştırıldı. Gereç ve Yöntem: Deri kanseri hücrelerine (B16F10) metformin uygulandı ve kaspaz-3, bcl-2, hafta 1, gadd153, grp78 ve AIF seviyeleri ELISA kullanılarak ölçüldü. Bulgular: Çalışmamızda metforminin cilt kanseri hücrelerinde apoptozis proteinlerini (caspaz-3, wee 1, gadd153, grp78 ve AIF) arttırdığı ve antiapoptotik markırı (bcl-2) azalttığı belirlendi. Sonuç: Çalışma sonuçları metforminin melanom cilt kanseri üzerine apoptotik etkisinin olduğunu gösterdiÖğe Protective effects of caffeic acid phenethyl ester, ellagic acid, sulforaphan and curcuma on malathion induced damage in lungs, liver and kidneys in an acute toxicity rat model(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2011) Alp, Harun; Aytekin, İsmail; Esen, Hasan; Alp, Ayşe; Büyükbaş, Sadık; Başaralı, Kemal; Hatipoğlu, Namık KemalThe aim of this study was to investigate the possible protective effects of caffeic acid phenethyl ester (CAPE), ellagic acid (EA), sulforaphan (SFN) and curcuma (CUR) against acute malathion (MAL) poisoning in rats. For that, 60 female adult Sprague-Dawley rats were randomly divided into 10 equal groups according to the treatment: whereas one group served as unmedicated control and another was intoxicated with malathion (200 mg/kg, per ox) and served as positive control, rats from the other groups were treated with each of the four antioxidants (CAPE: 10 mu mol/kg, intraperitoneally, EA: 85 mg/kg, per ox, SFN: 0.5 mg/kg, per os and CUR: 1 g/kg, per os) alone or in combination with malathion. One day later, serum AChE (acetylcholinesterase), amylase and GGT (gamma-glutamyltransferase) activities were determined and a histopathological evaluation was performed on lungs, kidneys and liver. In MAL-intoxicated rats, the AChE activity was markedly depleted whereas the GOT and amylase activities were significantly increased compared to the unmedicated controls. In parallel, severe and extended inflammatory and degenerative cell lesions were evidenced in liver, kidneys and lungs. By contrast, changes in the serum enzyme activities were greatly attenuated and the organ damage was also markedly reduced but not completely abrogated when an antioxidant cotreatment has been instituted. In addition, CUR appeared as the more efficient for hindering biochemical and histopathological alterations induced by malathion. These results show the protective effects of CAPE, EA, SFN and CUR on acute malathion poisoning in rats.
