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    Bacterial killing by several root filling materials and methods in an ex vivo infected root canal model
    (WILEY-BLACKWELL, 2011) Ozcan, E.; Eldeniz, A. U.; Ari, H.
    Ozcan E, Eldeniz AU, Ari H. Bacterial killing by several root filling materials and methods in an ex vivo infected root canal model. International Endodontic Journal, 44, 1102-1109, 2011. Aim To evaluate the ability of two root canal sealers (Epoxy resin-based AH Plus (R) or polydimethylsiloxane-based GuttaFlow (R)) and five root filling techniques (continuous wave of condensation, Thermafil (R), lateral condensation, matched taper single gutta-percha point, laterally condensed-matched taper gutta-percha point) to kill bacteria in experimentally infected dentinal tubules. Methodology An infected dentine block model was used. One hundred and twenty extracted, single-rooted human teeth were randomly divided into 10 test (n = 10) and 2 control (n = 10) groups. The roots, except negative controls, were infected with Enterococcus faecalis for 21 days. The root canals were then filled using the test materials and methods. Positive controls were not filled. Sterile roots were used as negative controls. Dentine powder was obtained from all root canals using gates glidden drills using a standard method. The dentine powder was diluted and inoculated into bacterial growth media. Total colony-forming units (CFU) were calculated for each sample. Statistical analysis was performed using the KruskalWallis and MannWhitney U test. Results The epoxy resin-based sealer was effective in killing E.faecalis except when using Thermafil (P < 0.05), but the polydimethylsiloxane-based sealer was not effective in killing this microorganism except in the continuous wave group (P < 0.05). Conclusions In the test model, AH Plus killed bacteria in infected dentine more effectively than GuttaFlow. The filling method was less important than the sealer material.
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    The influence of irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece
    (BLACKWELL PUBLISHING, 2007) Erdemir, A.; Eldeniz, A. U.; Ari, H.; Belli, S.; Esener, T.
    Aim To determine the influence of various irrigating solutions on the accuracy of the electronic apex locator facility in the Tri Auto ZX handpiece. Methodology One hundred and forty teeth with single canals and mature apices, scheduled for extraction for either periodontal or prosthetic reasons in 76 patients were used. Following informed written consent local anaesthesia was administered, access cavities were prepared and pulp tissue removed. The teeth were then randomly divided into seven groups according to the irrigating solutions used. The root canal length measurements were completed using the Tri Auto ZX handpiece with automatic reverse function in the presence of one or other of the following solutions: 0.9% saline, 2.5% NaOCl, 3% H2O2, 0.2% chlorhexidine, 17% EDTA, Ultracaine((R)) D-S or in the absence of an irrigating solution (control). Files were immobilized in the access cavity with composite resin. After extraction, the apical regions of the teeth were exposed and the file tips examined under a stereomicroscope. Distances between the file tips and the apical constriction were measured (mm) and analysed using a one-way ANOVA and post hoc Tukey test. Results Mean distances from the apical constriction to the file tip were longer in the 0.9% saline group (P < 0.05). There was no statistically significant difference on file tip position between the other solutions. Conclusions Tri Auto ZX gave reliable results with all irrigating solutions apart from in the presence of 0.9% saline.