Yazar "Arikan, Yueksel" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    The effects of CO2 pneumoperitoneum on the apoptotic index in the peritoneum
    (HEALTH COMMUNICATIONS INC, 2007) Tosun, Murat; Samli, Hale; Arikan, Yueksel; Solak, Mustafa; Sahin, Ali; Soeylemez, Zafer; Kalkan, Serpil
    During laparoscopic surgery, gases such as carbon dioxide (CO2), helium, or normal air are insufflated into the intra-abdominal cavity so the surgeon can obtain a clear surgical field; however, this insufflation technique may cause injury to the intra-abdominal organs. This study was undertaken to evaluate the effects of different pressures of CO2 on the apoptotic index in the peritoneum during laparoscopic surgery. A total of 30 Sprague-Dawley male rats were used in the study. CO2 was insufflated into the intra-abdominal cavity via an angiocatheter cannula by an insufflator at pressures of 10 and 20 mm Hg over 60 min. In the control group, the cannula was inserted into the intra-abdominal cavity, but no gas was insufflated. After 60 min, the rats were killed; peritoneum was harvested from the abdominal wall and was cultured in the cell culture laboratory. Apoptotic and living cells were detected immunohistochemically, and the apoptotic index was calculated and statistically analyzed. The data collected revealed that the apoptotic index increases in proportion to the level of CO2 pressure. CO2 pneumoperitoneum is a very useful technique. Gas pressure must be carefully set during the operation, however, or injured mesothelial cells may cause serious malfunction.
  • Küçük Resim Yok
    Öğe
    p53 expression and apoptosis in liver and spleen during CO2 pneumoperitoneum
    (SPRINGER, 2008) Arikan, Yueksel; Tosun, Murat; Saykol, Volkan; Kalkan, Serpil; Erdem, Serpil
    Backgrounds and aims Laparoscopic surgery techniques have been increasingly preferred to classic laparotomy by surgeons since 1987. However, this method has some important adverse effects on intra-abdominal organs. The aim of this study is to evaluate the effects of different pressures of CO2 on apoptosis and p53 expression in cells in liver and spleen. Methods In total, 30 male Sprague-Dawley rats were used in the study. CO2 was insufflated into the intra-abdominal cavity via angiocatheter cannule by an insufflator in two different pressures of 10 and 20 mm Hg for 60 min. However, in the control group, only cannule was inserted into the intra-abdominal cavity, but no gas was insufflated. After 60 min, the rats were killed and laparotomy was applied. The liver and spleen were excised. The samples were histologically processed and immunohistochemistry was applied. Results All the data revealed that the number of apoptotic cells in liver and spleen increases in proportion to CO2 pressure level. No p53 expression was detected in both organs. Conclusion CO2 pressure level and application time may affect on cells living in liver and spleen. High pressure and/or long application time may cause releasing of cytokines and superoxide radicals from these organs' cells, and transient or serious organ dysfunctions may occur.