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Öğe Anadolu merinoslarında sık kuzulatma olanaklarının araştırılması(2014) Bülbül, Bülent; Kırbaş, Mesut; Aktaş, Ahmet Hamdi; Köse, Mehmet; Ataman, Mehmet Bozkurt; Çoyan, Kenan; Kan, MustafaBu çalışmada sık kuzulatma yöntemi kullanılarak koyunlardan bir yılda elde edilecek kuzu sayısını ve buna bağlı olarak işletmenin karlılığını arttırma olanaklarının araştırılması amaçlanmıştır. Materyal olarak Özel sektörde Anadolu Merinosu ırkı 525 baş koyun ve 40 baş koç ve Bahri Dağdaş Uluslararası Tarımsal Araştırma Enstitüsü’nde (Kamu sektörü) 199 baş koyun ve 15 baş koç kullanıldı. Özel sektördeki ve Kamu sektöründeki koyunlar sık kuzulatma ve kontrol olmak üzere iki gruba ayrıldı. Özel sektörde 200, Kamu sektöründe ise 75 baş koyun normal olarak yılda bir kuzu elde etmek için kontrol olarak ayrılırken, Özel sektörde 325, Kamu sektöründe ise 124 baş koyun çalışma grubunu oluşturdu. Kontrol grubu koyunlar her yıl geleneksel tohumlama dönemi olan Ağustos-Eylül aylarında yılda bir çiftleştirilirken, çalışma grubunda bulunanlara 2 yılda 3 kuzulatma uygulandı. Çalışma grubu koyunlar ilk tohumlamadan itibaren 5 ay gebelik, 40 gün laktasyon ve 20 gün sütten kesmeyi takiben 1 aylık tohumlama periyoduna alındı. Çalışma grubunda sık kuzulatma için östrüs indüksiyonu ve senkronizasyonu amacıyla koç etkisi, koç etkisi flushing ve hormon uygulamalarını içeren beş farklı protokol kullanıldı. Sonuç olarak hormon kullanılan sık kuzulatma uygulamalarıyla yılda bir kuzulatmaya göre daha fazla kuzu verimi ve kuzu üretkenliği sağlandı. Sık kuzulatma uygulamalarında elde edilen kuzu verimi ve kuzu üretkenliği üzerinde uygulanacak senkronizasyon yöntemlerinin önemli derecede etkili olduğu görüldü. Ancak bu çalışmada uygulanan senkronizasyon yöntemleri ile elde edilen kuzu veriminin kontrol grubuna göre karlı olmadığı tespit edildiÖğe Combination of Cysteamine and Lipoic Acid Improves the Post-Thawed Bull Sperm Parameters(2016) Güngör, Şükrü; Aksoy, Adil; Yeni, Deniz; Avdatek, Fatih; Öztürk, Caner; Ataman, Mehmet Bozkurt; Coyan, KenanSunulan çalışmada sisteamin, trehaloz, alfa-lipoik asit ve bu antioksidan kombinasyonlarının, çözüm sonu boğa spermasında spermatolojik ve oksidatif stres parametreleri üzerine koruyucu etkinliklerinin belirlenmesi amaçlandı. Beş adet sağlıklı holştayn (3-4 yaşlarında) ırkı boğa kullanıldı. Çalışmada kullanılan her boğadan 8 ejakülat alındı. Alınan ejakülatlar miks yapılarak 37 C'de 7 eşit hacme bölündükten sonra, sisteamin 2 mM, trehaloz 50 mM, alfa-lipoik asit (ALA) 1 mM, sisteamin 2 mM trehaloz 50 mM, ALA 1 mM trehaloz 50 mM, sisteamin 2 mM ALA 1 mM ve antioksidan içermeyen (kontrol) temel sulandırıcı ile sulandırılarak 5 C'de soğutulmasının ardından donduruldu. Dondurulan payetler su banyosunda çözdürülerek değerlendirildi. Sisteamin 2 mM ALA 1 mM kombinasyonunu içeren sperma sulandırıcısının çözüm sonu subjektif (% 68 2.7) ve progresif motilite (% 42.9 4.7) oranları üzerine kontrol gruplarına kıyasla (% 61 4.2 ve % 37.5 8) olumlu etkinliği gözlenirken istatiksel olarak fark önemsiz bulundu (P 0.05). Sisteamin 2 mM ALA 1 mM kombinasyonu içeren sperma sulandırıcı grubu akrozom bütünlüğü ve mitokondriyal aktivite oranları (% 52.02 6.4 ve % 32 4.1) kontrol gruplarına (% 30.5 1.7 ve % 14.02 3.5) göre istatistiksel olarak daha yüksek bulundu (P 0.05). Dondurulmuş çözdürülmüş boğa sperma sulandırıcılarına eklenen sisteamin ve ALA kombinasyonu spermatozoon motilitesine, akrozom bütünlüğüne ve mitokondriyal aktivite bütünlüğüne katkı sağladı.Öğe Düvelerde Östrüsün Belirlenmesinde Kullanılan Pratik Yöntemlerin Karşılaştırılması(2002) Ataman, Mehmet Bozkurt; Kaya, Abdullah; Karaca, Fikret; Çoyan, KenanIn this study, detection of estrus was compared using the different estrus detection methods. A total of 19 Brown Swiss heifers aging between 18-24 months were used as materials. Heifers were examined two times with 10 days interval to detect ovarian activity. After that, a prostaglandin analogue of cloprostenol was injected two times with 11 days interval intramuscularly at a dose of 500 u.g per animal. The estrus cycle of each heifer was determined by observation of estrus signs, measurement of vaginal temperature and resistance, vaginal and transrectal examination after the second PGF2cl injections every 12 hr in a day for 4 days. Estrus detection rates were 63.1 % (observation of estrus signs), 36.8 % (vaginal temperature), 63.1 % (vaginal resistance), 63.1 % (vaginal examination) and 78.9 % (rectal examination), respectively. The differences of the estrus rates among the groups were not significant (p>0.05). As a conclusion, different estrus detection methods could be used to detect the estrus in heifers. Additionally, using of different method may prevent infertility resulting from suboestrus or inadequacy of estrus detection.Öğe Effect of Melatonin Implantation to Sperm Donor Rams on Post-Thaw Viability and Acrosomal Integrity of Sperm Cells in the Breeding and Non-Breeding Season(BLACKWELL WISSENSCHAFTS-VERLAG GMBH, 2001) Kaya, Abdullah; Aksoy, Melih; Başpınar, Nuri; Yıldız, Cengiz; Ataman, Mehmet BozkurtThe influence of melatonin administration to sperm donors on the freezability of ram semen and enzyme leakage through sperm cells during different steps of the cryopreservation process were evaluated in the breeding and non-breeding season. Melatonin implantation to rams in the breeding season improved post-thaw sperm viability and intact acrosome rates without influencing the motility rate (p < 0.05). Likewise, the post-thaw alkaline phosphatase release through sperm cells was significantly lower in the melatonin-treated group in comparison with untreated controls (p < 0.05). In the non-breeding season, melatonin administration enhanced intact acrosome rates (p < 0.05) and reduced aspartate aminotransferase activity (p < 0.05) postthaw in the offseason ejaculates. Melatonin implantation twice in the breeding and non-breeding season did not produce any further improvement in the post-thaw sperm parameters in the non-breeding season ejaculates. It was concluded that melatonin administration to sperm donors improved freezability of ram semen collected from these rams and reduced enzyme leakage through sperm cells during cryopreservation.Öğe Effect of Pgf(2 Alpha) Injection at Different Days of the Oestrous Cycle on the Interval to Oestrus and Ovulation and on Fertility in Ewes(Wiley-Blackwell, 2006) Ataman, Mehmet Bozkurt; Aköz, Mehmet[Abstract not Available]Öğe The effect of some seasonal conditions on oestrus occurrence in cows(ARCHIV FUR TIERZUCHT, 2009) Buelbuel, Buelent; Ataman, Mehmet BozkurtIn this study, the effect of climatic conditions on oestrus occurrence was investigated by using 9 972 oestrus records of cows recorded between 1995 and 2003. A distinct seasonal variation in the oestrus occurrence was determined. Oestrus occurrence observed in January, March, November and December was less than that in June and September (P<0.05). Annual distribution of the oestrus occurrence was positively correlated with environment temperature and insulation duration, but it was negatively correlated with rainfall (P<0.01). However, there was no relationship between oestrus occurrence and relative humidity. In addition to these, there was a slight decrease in the oestrus response when the temperature-humidity index (THI) was above 72; nevertheless, this decrease was not significant (P>0.05). The data presented in this study demonstrated that the increase in the environmental temperature up to 23 degrees C did not cause a suppressive effect on the ovarian activity. In conclusion, annual distribution of the oestrus occurrence is positively correlated with environment temperature and insulation duration whereas it is negatively correlated with rainfall in Holstein cows, in this study.Öğe EFFECTS OF ARGININE AND TREHALOSE ON POST-THAWED BOVINE SPERM QUALITY(AKADEMIAI KIADO ZRT, 2017) Ozturk, Caner; Gungor, Sukru; Ataman, Mehmet Bozkurt; Bucak, Mustafa Numan; Baspinar, Nuri; Ili, Pinar; Inanc, Muhammed EnesThe present study was conducted to examine the protective role of arginine and trehalose on post-thaw bull sperm and oxidative stress parameters. Five ejaculates for each bull were used in the study. Each ejaculate, split into three equal aliquots and diluted at 37 degrees C with base extenders containing 2 mM arginine, 25 mM trehalose and no antioxidant (control) was cooled to 5 degrees C and then frozen. Frozen straws were thawed in a water bath for evaluation. Supplementation of the semen extender with arginine decreased the percentages of post-thawed subjective motility (29 +/- 8.21%), CASA motility (12.2 +/- 5.69%) and progressive motility (3.52 +/- 2.13%), compared with the controls (43 +/- 2.73%, 55.4 +/- 6.78% and 33.48 +/- 4.14%, respectively, P < 0.05). Supplementation of the semen extender with trehalose produced a higher mitochondrial activity and sperm viability (36.3 +/- 3.99% and 44.1 +/- 2.18%) compared with the control (13 +/- 8.15 and 31.7 +/- 3.94%, respectively, P < 0.05). It was established that trehalose (95.1%) and arginine (92.8%) protect DNA integrity compared to the control (90.4%) (P < 0.05). Trehalose supplementation in semen extenders provided great benefit in terms of viability, mitochondrial activity, and intact sperm DNA on frozen-thawed bull sperm.Öğe Effects of Glucomannan on the Sacculus Rotundus and Peripheral Blood Lymphocytes in New Zealand Rabbits during Aflatoxicosis(HINDAWI LTD, 2012) Sur, Emrah; Donmez, Hasan Huseyin; Boydak, Murat; Ataman, Mehmet BozkurtThis study was aimed to determine the effects of the glucomannan added to aflatoxin- (AF-) contaminated diet on the sacculus rotundus and peripheral blood lymphocytes of New Zealand rabbits by histological and enzyme histochemical methods. Twenty-four adult rabbits of both sexes were divided into four equal groups, namely, as control, glucomannan 0.2 g/day, AF 125 mu g/kg/day, and glucomannan combined with AF. The animals in all groups were treated for 12 weeks by the above-mentioned diet. When compared to control, AF-treatment caused significant decrease in alpha-naphthyl acetate esterase- (ANAE-) positive peripheral blood lymphocyte (PBL) percentages. The addition of the glucomannan to AFcontaining diet recovered the adverse effects of AF on sacculus rotundus and increased the ANAE-positive PBL counts. These results suggested that glucomannan was effective against the negative effects of AF in rabbits.Öğe Esterified glucomannan improves aflatoxin-induced damage of sperm parameters during liquid storage of ram semen at 5 degrees C(ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014) Ataman, Mehmet Bozkurt; Bucak, Mustafa Numan; Coyan, KenanThe aim of the present work was to study the effects of aflatoxin (AF) on sperm parameters in rams, and to determine the protective efficiency of esterified glucomannan (EG) co-administered with AF up to 96 h of the liquid storage of ram semen at 5 degrees C. Thirty-two Merino rams (12-14 months old) were used. The animals were examined for their general health status. To ensure their adaptation to the environment and the new feeding regimen, a 15-day acclimatization programme was applied to the animals, prior to the start of the study. Experimental feeding was continued for ninety-two days. The experimental design consisted of four dietary treatments. The control group (C) was fed with commercial feed. The AF group was fed with commercial feed plus 250 mu g/day of total AF. The EG group received commercial feed plus 2 g/day of EG. The AF + EG group was given commercial feed plus 250 mu g/day of total AF and 2 g/day of EG. In the study, ejaculates were obtained from rams twice a week for 12 weeks, using an electro-ejaculator. After collected, the ejaculates were diluted with a skimmed milk extender, and stored at 5 degrees C. Sperm motility and rates of abnormal and nonviable spermatozoa were determined for the different treatment groups at 5 degrees C at 0, 24, 48, 72 and 96 h of liquid storage. During the first two weeks of the trial, the groups did not statistically differ from each other for sperm motility or rates of abnormal and nonviable spermatozoa at 0, 24, 48 and 96 h of storage. As from the third week, the short-term storage of semen produced statistically significant differences between the AF group and the other treatment groups for sperm parameters (p < 0.05). The administration of aflatoxin was observed to have reduced sperm motility and to have increased the rates of abnormal and nonviable spermatozoa in comparison to the control group (p < 0.05), while EG co-administered with AF was determined to have ameliorated the adverse effects of AF on sperm parameters, and this ameliorative effect continued throughout the short-term storage of semen. On the other hand, aflatoxin administration resulted in the deterioration of the sperm parameters in the following weeks, and the combined administration of EG + AF reversed this adverse effect, thus, bringing the sperm parameters closer to the values of the control group. This study demonstrated that, in rams, AF adversely affected sperm, biochemical and testis parameters, and that the combined administration of EG and AF reversibly improved these adverse effects. (C) 2014 Elsevier Inc. All rights reserved.Öğe Estimation of early pregnancy by electrical resistance values of vaginal mucosa in cows and heifers(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2005) Taşal, İbrahim; Ataman, Mehmet Bozkurt; Aksoy, Melih; Kaya, Abdullah; Karaca, F; Tekeli, TevfikThe aim of this study was to investigate the availability of vaginal resistance values to estimate early pregnancy in heifers and cows on days 18-21 post-insemination. Seven Brown Swiss cows and ten heifers without any palpable reproductive disorders were used. Cloprostenol, a synthetic analogue of PGF(2 alpha), was administered intramuscularly twice (500 mu g) 11-days apart. After second cloprostenol administration, animals were observed for the overt signs oestrus and, inseminated twice with frozen semen after confirmation of oestrus by rectal palpation. Electrical conductivity of vaginal mucosa was measured through ventral wall of vagina adjacent to caudal end of cervix uteri by a handy-held instrument. Vaginal conductance of animals was measured twice, with a 12 h interval, on days 0 (day of oestrus), 3, 6, 10, 11 19 20 and 21 in cows, and on days 0 (day of oestrus), 3, 6, 10, 13, 18, 19 and 20 in heifers. To estimate plasma progesterone levels, jugular blood samples (10 mL) were also collected on days 19, 20 and 21 for cows, and 18, 19 and 20 for heifers. Pregnant animals were detected by rectal palpation 60 days after insemination. In pregnant cows, electrical resistance of vaginal mucosa measured between days 19-21 was significantly higher than non-pregnant animals, while vaginal resistance values determined on the day of insemination, and on days 3, 6, 10 and 13 post-insemination were similar in both groups. Likewise, vaginal impedance readings in pregnant heifers determined between days 18-20 were significantly higher than those non-pregnant animals. Vaginal resistance measurements in cows (between days 19-21) and heifers (between days 18-20) were significantly correlated with plasma progesterone concentrations both in pregnant and non-pregnant groups. It was concluded that vaginal resistance values might be used for the diagnosis of early pregnancy as a contributor or predictor method in cows and heifers.Öğe Estrus synchronization using short- and long-term proestagen treatments and ram effect and ram effect plus flushing in ewes outside the breeding season(WILEY-BLACKWELL, 2011) Bülbül, Bülent; Kırbaş, Mesut; Ataman, Mehmet Bozkurt; Coyan, K.; Akbulut, Kerem; Köse, M.; Halıcı, I.[Abstract not Available]Öğe Geçiş dönemi başındaki akkaraman melezi koyunlarda farklı dozda flourogestene acetate, norgestomet ve PGF2? ile senkronize östrüslerin uyarılması(2009) Ataman, Mehmet Bozkurt; Aköz, Mehmet; Fındık, Murat; Saban, ErdalBu çalışmada, geçiş dönemi başındaki koyunlarda farklı progesteron ve PGF2? uygulamalarının, östrüs senkronizasyonu ve ovaryum aktivitesini uyarmadaki etkisi araştırıldı. Araştırmada toplam 75 koyun kullanıldı ve koyunlar 5 gruba ayrıldı. Birinci gruptaki koyunlara (n15) 30 mg flourogesterone acetate (FGA-30), ikinci gruptaki koyunlara (n15) 40 mg flourogestene acetate (FGA-40) içeren süngerler vagina içine yerleştirildi. Her iki gruptaki süngerler 12 gün sonra uzaklaştırıldı. Üçüncü gruptaki koyunlara (n15) 3 mg Norgostomet içeren kulak implantı (N-İMPLANT) kulak derisi altına yerleştirildi ve 9 gün sonra uzaklaştırıldı. Dördüncü gruptaki koyunlara (n15) Tiaprost tromethamine (PGF2?; 0.294 mg) 9 gün arayla iki kez kas içi yolla enjekte edildi. Beşinci grup (n15) kontrol olarak oluşturuldu. Vaginal süngerlerin, implantların çıkarılmasını ve ikinci PGF2? enjeksiyonunu takiben tüm çalışma gruplarındaki koyunlara kas içi yolla 600 IU PMSG enjekte edildi. Östrüste oldukları belirlenen koyunlara doğal aşım yaptırıldı. Östrüs görülme oranları FGA-30 ve N-İMPLANT gruplarında (%93.3), FGA-40 grubunda (%86.6) ile PGF2? (%53.3) ve Kontrol (%26.6) gruplarına göre istatistiki açıdan önemli şekilde yüksek belirlendi. Gebelik oranları ise FGA-30 (%93.3) ve N-İMPLANT (%93.3) gruplarında, PGF2? (%53.3) grubuna göre belirgin olarak yüksek bulundu. Kuzulama oranı açısından deneme grupları arasında önemli bir farklılık gözlenmedi. Çoklu doğum oranı N-İMPLANT grubunda (%35.7), PGF2? (%0.0) grubuna göre belirgin olarak yüksek bulundu. Sonuç olarak, geçiş dönemi başındaki koyunlarda PGF2?‘nın çift doz uygulanmasıyla farklı progesteron tedavileri kıyaslandığında PGF2?‘nın yeterli cevap oluşturamadığı belirlendi.Öğe GNRH-PGF(2 alpha) and PGF(2 alpha)-PGF(2 alpha) synchronization in Akkaraman cross-bred sheep in the breeding season(NATL VETERINARY RESEARCH INST, 2006) Ataman, Mehmet Bozkurt; Aköz, MehmetThe object of this trial was to determine the efficacy of GnRH-PGF(2 alpha) treatment to synchronize oestrus in sheep during the breeding season. A total of 30 ewes aging 18-24 months and 6 healthy rams aging 2-3 years were used in this experiment. Animals were divided into two groups. The first group received intramuscular (i.m.) injection of GnRH analogue (busereline) and 5 d later i.m. injection of PGF(2 alpha) analogue (Triaprost tromethamine). The second group (control) was given twice, at 9 d interval, i.m. injection of PGF(2 alpha). Fourty-eight hours after the application of PGF(2 alpha) 400 lU of PMSG was injected i.m. to all the ewes. After the detection of oestrous, the ewes were hand-mated naturally. Pregnancy was determined using ultrasonography on d 30 after the mating. Multiple embryos were determined in each ewe using ultrasonography. Oestrus response, pregnancy, lambing rates, and litter sizes were 93.7%, 85.7%, 83.3% and 1.70; 86.6%, 84.6%, 81.8%, and 1.66 in the groups I and II, respectively. Therefore, GnRH-PGF(2 alpha) treatment has been found to be effective in the synchronization of the oestrus in ewes.Öğe GNRH-PGF2α and PGF2α-PGF2α synchronization in Akkaraman cross-bred sheep in the breeding season(2006) Ataman, Mehmet Bozkurt; Aköz, MehmetThe object of this trial was to determine the efficacy of GnRH-PGF 2? treatment to synchronize oestrus in sheep during the breeding season. A total of 30 ewes aging 18-24 months and 6 healthy rams aging 2-3 years were used in this experiment. Animals were divided into two groups. The first group received intramuscular (i.m.) injection of GnRH analogue (busereline) and 5 d later i.m. injection of PGF2? analogue (Triaprost tromethamine). The second group (control) was given twice, at 9 d interval, i.m. injection of PGF2?. Fourty-eight hours after the application of PGF2?, 400 IU of PMSG was injected i.m. to all the ewes. After the detection of oestrous, the ewes were hand-mated naturally. Pregnancy was determined using ultrasonography on d 30 after the mating. Multiple embryos were determined in each ewe using ultrasonography. Oestrus response, pregnancy, lambing rates, and litter sizes were 93.7%, 85.7%, 83.3% and 1.70; 86.6%, 84.6%, 81.8%, and 1.66 in the groups I and II, respectively. Therefore, GnRH-PGF2? treatment has been found to be effective in the synchronization of the oestrus in ewes.Öğe Induction of multiple births in Akkaraman cross-bred sheep synchronized with short duration and different doses of progesterone treatment combined with PMSG outside the breeding season(NATL VETERINARY RESEARCH INST, 2006) Aköz, Mehmet; Bülbül, Bülent; Ataman, Mehmet Bozkurt; Dere, SevgiDetermination of the optimal PMSG dose for inducing increased the prolificacy in Akkaraman cross-brcd ewes synchronized with the different doses of fluorogestone acetate (FGA) outside the breeding season was the aim of this study. A total of 90 non-lactating ewes were randomly divided into two groups. Vaginal sponges containing 30 mg (group FGA1) and 40 mg (group FGA2) of fluorogestone acetate were inserted into the vagina of the ewes. The sponges were withdrawn on day 7 and 300 IU of PMSG (FGA1A, FGA2A), 500 IU of PMSG (FGA1B, FGA2B) and 700 IU of PMSG (FGA1C, FGA2C) were injected intramuscularly in the named above subgroups of FGA1 and FGA2. Oestrus response, pregnancy, lambing, and multiple birth rates were 100%, 93.3%,78.6% and 18.8% in the group FGA1A, 93.3%, 92.8%, 76.9% and 40% in the group FGA1B, 100%, 100%, 86.7% and 69.2% in the group FGA1C, 93.3%, 92.8%, 76.9% and 20% in the FGA2A, 92.8%, 100%, 84.6% and 36.4% in the group FGA2B, 100% 93.3%, 85.7%, 66.7% in the group FGA2C, respectively. Multiple birth rates in the groups FGA1C and FGA2C were significantly higher than in the other groups. In conclusion, the application of 700 IU PMSG was rather more effective than injections of 300 IU and 500 IU in ewes being outside the breeding season.Öğe Induction of synchronized oestrus in akkaraman cross-bred ewes treated with prostaglandine F2 alpha, norgestomet and sponges impregnated with different doses of fluorogestene acetate at the beginning transitional period(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2009) Ataman, Mehmet Bozkurt; Aköz, Mehmet; Fındık, Murat; Şaban, ErdalThe efficiency of different progesterone and PGF2 alpha treatments in the induction of ovarian activity and synchronization of oestrus was investigated the beginning transitional period in sheep. A total of 75 ewes were used in the experiment. Animals were divided into 5 groups. Vaginal sponges containing either 30 mg or 40 mg fluorogestene acetate (FGA) were inserted into the vagina of ewes in the first (FGA-30; n=15) and second (FGA-40; n=15) groups, respectively. The sponges were withdrawn after 12 day. In the third group of ewes (n=15), ear implants containing 3 mg norgestomet (N-IMPLANT) were inserted subcutaneously and removed after 9 day. In the fourth group (n=15), Tiaprost tromethamine (PGF2 alpha; 0.294 mg) was intramuscularly injected twice at an interval of 9-d. The control (Control) group is consisted of 15 ewes. After the second PGF2 alpha injections and the withdrawal of the sponges and implants, 600 IU PMSG was injected to all ewes in the FGA-30, FGA-40, N-Implant and PGF2 alpha groups. After the detection of oestrus ewes, they were naturally mated. Oestrus response rates were significantly higher in the groups FGA-30 (93.3%), and N-IMPLANT (93.3%) than those in the groups PGF2 alpha (53.3%) and Control (26.6%). However, oestrus rates in the group FGA-40 were only significantly higher (86.6%) than those in the group Control (26.6%). The pregnancy rates in the groups FGA-30 (93.3%) and N-IMPLANT (93.3%) were significantly higher than that in the group PGF2 alpha (53.3%). No significant difference was observed among the groups FGA-30, FGA-40, N-IMPLANT and PGF2 alpha with respect to lambing rates. Multiple birth rates were significantly higher in the group N-IMPLANT (35.7%) than in the group PGF2 alpha (0.0%). As a conclusion, a double dosed application of PGF2 alpha was observed to be inefficient compared to different progesterone treatments in ewes the beginning transitional period.Öğe İneklerde östrüs senkronizasyonu(2005) Bülbül, Bülent; Ataman, Mehmet Bozkurtneklerde östrüslerin doğru belirlenebilmesi ve bu işe harcanan zamanın azaltılabilmesi yönünde yoğun çalışmalar yapılmaktadır. Son yıllarda bu amaç için, değişik hormon preparatlarının tek başına veya kombine kullanıldığı östrüs senkronizasyon programları geliştirilmiştir. Sabit zamanlı tohumlamaların birlikte kullanıldığı östrüs senkronizasyon programları uygulama kolaylığı, sakin kızgınlık gösteren hayvanların tohumlanmasına olanak sağlaması, östrüs tespiti için harcanan zaman ve işgücü kaybını önlemesinden dolayı saha şartlarında tercih edilmektedir. Suni tohumlama uygulamasıyla birlikte kullanılan bu senkronizasyon programlarının saha uygulamaları sırasında avantaj ve dezavantajlarının ortaya konularak, en uygun yöntemin seçilmesi, sığır populasyonundan en üst düzeyde faydalanılması açısından oldukça önemlidir.Öğe Influence of Methionine and Dithioerythritol on Sperm Motility, Lipid Peroxidation and Antioxidant Capacities During Liquid Storage of Ram Semen(Elsevier Sci Ltd, 2010) Çoyan, Kenan; Başpınar, Nuri; Bucak, Mustafa Numan; Akalın, Pınar Peker; Ataman, Mehmet Bozkurt; Ömür, Ali Doğan; Güngör, Şükrü; Küçükgünay, Sadık; Özkalp, Birol; Sarıözkan, SerpilThe aim of this study was to investigate the effects of methionine and dithioerythritol, added to the Tris extender, on ram sperm motility and LPO (lipid peroxidation) and antioxidant capacities during liquid storage up to 72 h at 5 degrees C. Ejaculates collected from five Merino rams, were evaluated and pooled at 37 degrees C. This study included two experiments. In experiment 1, each pooled ejaculate was divided into four equal aliquots and diluted (37 degrees C) with the base extender, containing 0 (control), 1, 2 and 4 mM methionine, at a final concentration of approximately 4 x 10(8) sperms/ml (single step dilution), in a 15-ml plastic centrifuge tube. In experiment 2, dithioerythritol, at concentrations of 0 (control). 0.5, 1 and 2 mM, was used as an additive in the extender, and the procedure explained above was applied for the division of aliquots and the dilution of semen. Diluted semen samples were kept in glass tubes and cooled from 37 to 5 degrees C in a cold cabinet, and maintained at 5 degrees C. Sperm motility and LPO and total glutathione (GSH) and glutathione peroxidase (GPx) capacities were determined at 5 degrees C for periods of 0, 24,48 and 72 h of liquid storage. The extender supplemented with 1 mM methionine led to higher motility percentages (77.0 +/- 1.2%), in comparison to the control group (66.0 +/- 4.9%), during 72 h of liquid storage (P < 0.05). As regards dithioerythritol, it did not statistically improve the motility rates for any of the storage times at 5 degrees C. In biochemical assays, differences in LPO levels between the groups with antioxidants and the control groups were not statistically significant. Compared to the control group, no significant difference was observed in GSH and GPx activities following the addition of methionine, during 72 h of storage. Total GSH and GPx activities did not increase significantly upon supplementation with 0.5 and 1 mM of dithioerythritol, compared to the control group, at any of the time points (P > 0.05). Dithioerythritol at 2 mM led (P < 0.01) to elevating GSH activity, compared to the control group, during 72 h of liquid storage. GPx activity was approximately 10 times higher for 2 mM of dithioerythritol (P < 0.001), compared to that of the control group at all time points. The question regarding the sustainability of sperm survival, LPO and antioxidant capacities following liquid storage of semen remains unanswered. Further studies are required for a better understanding of the biochemical changes and to obtain more information on the determination of lipid peroxidation and antioxidant capacities during cooled storage of ram semen.Öğe Investigation of accelerated lambing possibility of anatolian merino sheep(KAFKAS UNIV, VETERINER FAKULTESI DERGISI, 2014) Bülbül, Bülent; Kırbaş, Mesut; Aktaş, Ahmet Hamdi; Köse, Mehmet; Ataman, Mehmet Bozkurt; Çoyan, Kenan; Kan, MustafaInvestigation of the possibilities to increase the number of lamb gained in a year via using the accelerated lambing method and, the profitability of a farm related to this is aimed with this study. As material, 525 Anatolian Merino ewes and 40 rams, aged at 2-4, in field conditions, and 199 ewes and 15 rams at Bahri Dagdas International Agricultural Research Institute were used. Ewes in the field condition and at the Institute were divided in to two groups as accelerated lambing and control and, 200 ewes in the field condition and 75 ewes at the Institute were remained as control to get one lamb per year while 325 in the field condition and 124 at the Institute were formed treatment group. The control ewes were bred in August and September, the traditional breeding season, in a 12 month interval while 3 lambings in 2 years were applied to the ewes in the treatment group and they were bred for one month again following the period of 5 months of pregnancy, 40 days of lactation and 20 days of weaning. Ram effect, ram effect + flushing and some different protocols were used for induction and synchronization of estrus for accelerated lambing in the treatment group. As a result, more fecundity and lamb productivity achieved by accelerated lambing than once a year lambing. Synchronization methods were found to be effective on fecundity and lamb productivity in accelerated lambing applications. However, lamb yield obtained by synchronization methods used in this study were not profitable.Öğe Koç spermasının ekilibrasyon ve dondurma-çözdürme sonrası spermatolojik biyokimyasal parametreleri üzerine lipoik asitin etkisi(Selçuk Üniversitesi, 2011) Başpınar, Nuri; Çoyan, Kenan; Bucak, Mustafa Numan; Ömür, Ali Doğan; Ataman, Mehmet Bozkurt; Akalın, Pınar Peker; Güngör, Şükrü; Öztürk, CanerAmaç: Koç sperması sulandırıcısına katılan farklı dozlardaki lipoik asitin spermanın ekilibrasyon ve dondurma sonrasi bazı spermatolojik ve biyokimyasal parametreler üzerine etkilerinin araştırılması amaçlandı. Gereç ve Yöntem: Araştırmada 6 baş ergin Konya Merinosu koçlara ait ejakülatlar kullanıldı. Ejakülatlar 4 eşit hacme bölünerek lipoik asit 1, 2, 4 mM içeren ve içermeyen (kontrol) Tris sulandırıcısıyla ml'de yaklaşık 400 x 10° spermatozoa olacak şekilde 37 °C'ta sulandırıldı ve donduruldu. Bulgular: Ekilibrayon sonra lipoik asitin kontrol grup larina göre sperm parametrelerinde etkisi görüimerken, dondurma-condarme sonrası numunelerde lipoik asit 1 mM (%743), kontrol (625) ve lipoik asit 4 mM (650) gruplara göre motilite yönünden önemli derecede iyileş tirme sağladı (p<0.001) Spermatoscanin HOS test ve ak botinluğu üzerinde dondurma-çözdürme sonrau tipasti etkinliği görülmeda Lipnik asitin 1, 2 ve 4 mM doslar ekilibrasyon ve dondurma sonra biyokimyasal parametreler yöminden kontrol gruplarına göre etkinlikleri beli bulunmadı (p-0.05) Öneri: Koç spermasına 1mM dozda lipoik asit ilavesi dondurma-çözdürme sonrası motiliteyi arttırmaktadır. Bu- nunla birlikte lipoik asitin farklı sulandırıcılarda ve hayvan türleri-ırklarında doz denemelerinin yapılması gerekmektedir.
