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    Effect of Different Storage Temperatures on the Stability of Bovine Viral Diarrhea Virus RNA in Blood Samples
    (UNIV AGRICULTURE, FAC VETERINARY SCIENCE, 2015) Avci, Oguzhan; Bulut, Oya; Yapici, Orhan; Simsek, Atilla; Yavru, Sibel; Dik, Irmak; Atli, Kamil
    The present study was conducted to determine stability of Bovine viral diarrhea virus (BVDV) RNA stored at different temperatures. A total of 6 blood samples obtained from a private cattle farm, which were found to be antigen positive (Ag+) by direct ELISA method, were used in this study. BVDV Ag+ samples were stored separately at 4, 21 and 37 degrees C for 1 month. The samples were analyzed on the 0, 1(st), 2(nd), 3(rd) and 4(th) weeks by ELISA for the presence of BVDV Ag and by RT-PCR for the presence of BVDV RNA. Stability of BVDV RNA was calculated using maximum concentration (C-max) and area under the curve (AUC) as kinetic parameters of each sample. All of the samples were found positive both by ELISA and RT-PCR on each week. Cmax values of BVDV RNA for the storage temperatures of 4, 21 and 37 degrees C were 356, 346 and 338 ng/mu L respectively, and AUC(0 -> 4) values for the same temperatures were 1151, 1106 and 1077 week. ng/mu L respectively. It was determined that storage at different temperatures for one month does not statistically influence the kinetic parameters of BVDV RNA (P>0.05). In conclusion, it can be expressed that storage of BVDV RNA at 4, 21 and 37 degrees C for one month has no effect on the stability of BVDV RNA. (C) 2015 PVJ. All rights reserved
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    Matrix metalloproteinases 2 and 9 activity after intra-articular injection of autologous platelet-rich plasma for the treatment of osteoarthritis in dogs
    (VETERINARNI A FARMACEUTICKA UNIVERZITA BRNO, 2018) Arican, Mustafa; Simsek, Atilla; Parlak, Kurtulus; Atli, Kamil; Sonmez, Gonca
    Intra-articular injection of platelet-rich plasma offers a promising potential for treatment of osteoarthritis in dogs. Twenty dogs weighing 25 to 50 kg (mean 38 kg) with unilateral stifle osteoarthritis were used for the study. Fourteen dogs were given intra-articular platelet rich plasma treatment and 6 dogs were used as controls. Double centrifuge method was used to obtain platelet-rich plasma. Radiography and ultrasonography of the affected joint were carried out and scores for lameness severity and pain severity were assigned by the attending clinicians. Synovial fluid was collected under sterile conditions at pre-treatment and on the 1st, 3rd, 5th, 7th, 15th days, and 4, 8 and 12 weeks after treatment. Gelatin zymography and Enzyme-linked Immunosorbent Assay were used to determine the synovial fluid levels of matrix metalloproteinase-2 (MMP-2) and metalloproteinase-9 (MMP-9). No adverse effects associated with the injection of the platelet concentrate or saline were observed. Hudson Visual Analog Scale and Canine Brief Pain Inventory scores for all components were non-significantly different between weeks 0, 4, 12 for control dogs. Matrix metalloproteinase-9 was totally and MMP-2 was partially inhibited in the platelet-rich plasma group. In the control group, MMP-9 was partially inhibited during the first month and activation started later. Matrix metalloproteinase-2 was constant in control samples throughout the experimental period. Platelet-rich plasma is a safe and effective method for treatment of dogs with osteoarthritis, possibly more useful for early cases with mild and moderate osteoarthritis. It is suggested that plasma rich platelet should be injected several times at regular intervals instead of a single application.
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    The Serological and Virological Investigation of Canine Adenovirus Infection on the Dogs
    (HINDAWI LTD, 2013) Bulut, Oya; Yapici, Orhan; Avci, Oguzhan; Simsek, Atilla; Atli, Kamil; Dik, Irmak; Yavru, Sibel
    Two types of Canine Adenovirus (CAVs), Canine Adenovirus type 1 (CAV-1), the virus which causes infectious canine hepatitis, and Canine Adenovirus type 2 (CAV-2), which causes canine infectious laryngotracheitis, have been found in dogs. In this study, blood samples taken from111 dogs, which were admitted to the Internal Medicine Clinic of Selcuk University, Faculty of Veterinary Medicine, with clinical symptoms. Seventy-seven dogs were sampled from Isparta and Burdur dog shelters by random sampling, regardless of the clinical findings. Dogs showed a systemic disease, characterized by fever, diarrhea, vomiting, oculonasal discharge, conjunctivitis, severe moist cough, signs of pulmonary disease and dehydration. Two dogs had corneal opacity and photophobia. In serological studies, 188 serum samples were investigated on the presence of CAV antibodies by ELISA. Total 103 (103/188-54.7%) blood samples were detected to be positive for CAV antibodies by ELISA. However, 85 (85/188-45.2%) blood samples were negative. Blood leukocyte samples from dogs were processed and inoculated onto confluent monolayers of MDCK cells using standard virological techniques. After third passage, cells were examined by direct immunoflourescence test for virus isolation. But positive result was not detected. In conclusion, this study clearly demonstrates the high prevalence of CAV infection in dogs.